{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T10:49:55Z","timestamp":1770720595550,"version":"3.49.0"},"reference-count":22,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,11,17]],"date-time":"2005-11-17T00:00:00Z","timestamp":1132185600000},"content-version":"vor","delay-in-days":6895,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1987,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The major goal of the present study was to develop a limiting dilution system for the enumeration of T cells which respond to mycobacterial antigens. Purified T cells from <jats:italic>M. tuburculosis<\/jats:italic>\u2010immune mice were restimulated with mycobacterial antigens and accessory cells, and after 4 days expanded with antigen, accessory cells and T cell growth factor. After another 3 days, proliferative responses were determined. Similar cultures performed without antigen served as controls. Limiting dilution analysis revealed that approximately 1\/2000 to 1\/3000 T cells from <jats:italic>M. tuberculosis<\/jats:italic>\u2010immune mice responded to whole <jats:italic>M. tuberculosis<\/jats:italic> organisms while T cells from normal mice did not respond. Similar T cell numbers reacted with several mycobacterial strains indicating expression of shared T cell antigens. Using a semi\u2010purified recombinant 64\u2010kDa protein from <jats:italic>M. bovis<\/jats:italic> the frequency of T cells generated after immunization with <jats:italic>M. tuberculosis<\/jats:italic> which reacted with a single mycobacterial protein could be estimated. We found that approximately 1\/5 of the <jats:italic>M. tuberculosis<\/jats:italic>\u2010reactive T cells recognized this particular antigen. Immunization with the recombinant 64\u2010kDa protein in an adjuvant containing trehalose dimycolate, monophosphoryl lipid A and mycobacterial cell wall skeleton stimulated an equally high number of <jats:italic>M. tuberculosis<\/jats:italic>\u2010reactive T cells (1\/2000). These findings demonstrate (a) that a high proportion of tuberculosis\u2010responsive T cells are directed against the 64\u2010kDa protein and (b) that immunization with this antigen in an appropriate adjuvant system is capable of stimulating high numbers of <jats:italic>M. tuberculosis<\/jats:italic>\u2010reactive T cells. Limiting dilution analysis with a panel of mycobacterial proteins or peptides may allow their ranking from immunodominant to immunosilent and facilitate identification of antigens or epitopes relevant to protection.<\/jats:p>","DOI":"10.1002\/eji.1830170308","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T00:30:33Z","timestamp":1172709033000},"page":"351-357","source":"Crossref","is-referenced-by-count":159,"title":["Enumeration of T cells reactive with <i>Mycobacterium tuberculosis<\/i> organisms and specific for the recombinant mycobacterial 64\u2010kDa protein"],"prefix":"10.1002","volume":"17","author":[{"given":"Stefan H. E.","family":"Kaufmann","sequence":"first","affiliation":[]},{"given":"Ulla","family":"V\u00e4th","sequence":"additional","affiliation":[]},{"given":"Jelle E. R.","family":"Thole","sequence":"additional","affiliation":[]},{"given":"Jan D. A.","family":"Van Embden","sequence":"additional","affiliation":[]},{"given":"Frank","family":"Emmrich","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"779","volume":"61","author":"Assaad F.","year":"1983","journal-title":"Bull. WHO."},{"key":"e_1_2_1_3_2","first-page":"349","volume":"70","author":"Anonymus","year":"1979","journal-title":"Indian J. Med. 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