{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T05:12:29Z","timestamp":1770700349852,"version":"3.49.0"},"reference-count":40,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,11,17]],"date-time":"2005-11-17T00:00:00Z","timestamp":1132185600000},"content-version":"vor","delay-in-days":6895,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1987,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We studied the activation of small resting mouse T lymphocytes by antibodies to the T cell antigen receptor in combination with antibodies to other T cell surface antigens. Solid\u2010phase but not soluble antibodies KJ16\u2010133 and F23.1, both directed to \u03b2 chains of the V<jats:sub>\u03b2<\/jats:sub>8 family, activate T cells to proliferate in the presence of growth factors, in a dose\u2010dependent fashion. Antibodies to Lyt\u20102 and to L3T4 had no activating effect at any concentration. However, submitogenic concentrations of KJ16\u2010133 and of F23.1 synergized with a wide range of concentrations of anti\u2010Lyt\u20102 and anti\u2010L3T4 to cause T cell proliferation similar or greater in magnitude to that caused by high concentrations of anti\u2010T cell receptor antibody. Synergistic activation was also observed with antibodies to Lyt\u20101, LFA\u20101 and H\u20102 class I antigens but to a significantly lower degree. This was particularly clear in limiting dilution experiments in which the corrected frequencies of T cells proliferating in response to low amounts of anti\u2010T cell receptor antibody together with anti\u2010Lyt\u20102 were 1\/4 to 1\/7 for BALB\/c T cells. The frequencies of BALB\/c T cells responding to high concentrations of anti\u2010T cell receptor antibody alone were between 1\/14 and 1\/126 and still lower frequencies of T cells proliferated in synergistic responses with anti\u2010LFA\u20101 or anti\u2010Lyt\u20101. Synergistic activation leads to the induction of functional cytotoxic cells. We interpret these data as suggestive that cross\u2010linking of the T cell antigen receptor with either Lyt\u20102 (CD8) or L3T4 (CD4) represents an optimal activating signal for resting T cells. We think that, in physiological T cell activation, cross\u2010linking of the T cell receptor to CD8 or CD4 is induced by their simultaneous binding to major histocompatibility complex (MHC) class I (for CD8) or MHC class II (for CD4) molecules on stimulator cells. We consider the possibility that similar cross\u2010linking requirements may also exist during T cell repertoire selection in ontogeny, thus accounting for the strict coexpression of MHC class I and class II\u2010restricted T cell receptors with CD8 and CD4 molecules, respectively.<\/jats:p>","DOI":"10.1002\/eji.1830170510","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T00:40:13Z","timestamp":1172709613000},"page":"643-650","source":"Crossref","is-referenced-by-count":87,"title":["Effective activation of resting mouse T lymphocytes by cross\u2010linking submitogenic concentrations of the T cell antigen receptor with either Lyt\u20102 or L3T4"],"prefix":"10.1002","volume":"17","author":[{"given":"Klaus","family":"Eichmann","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan\u2010Ingvar","family":"J\u00f6nsson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ingrid","family":"Falk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank","family":"Emmrich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6606228"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"1714","DOI":"10.4049\/jimmunol.135.3.1714","volume":"135","author":"Schwab R.","year":"1985","journal-title":"J Immunol."},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830160310"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/317627a0"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.160.2.452"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.161.5.1219"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1983.tb01100.x"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.161.6.1450"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.162.4.1393"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.157.2.705"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.158.3.836"},{"key":"e_1_2_1_13_2","volume-title":"The generation of the repertoire of T cell specificities and functions: towards a consistent model, Progr. 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