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Fifteen hybridomas were productive, and one (RS\u20103.1) was cloned and the affinity\u2010purified product analyzed for its reactivity pattern by MOPC 104E\u2010enzyme\u2010linked immunosorbent assay inhibition. Among five BALB\/c myelomas only TEPC 183 (IgM) was active, not those belonging to other Ig classes. Among normal sera from 8 mouse strains only those of BALB\/c, DBA\/2J and CBA\/J showed inhibition.<\/jats:p><jats:p>The recombinant strain BALB\u2010Igh\u2010V<jats:sup>a<\/jats:sup>\/Igh\u2010C<jats:sup>b<\/jats:sup> did not react, which shows that its C<jats:sub>\u03bc<\/jats:sub> stems from parental strain C57BL\/6, and that therefore the recombination event had occurred 5\u2032 of this gene.<\/jats:p>","DOI":"10.1002\/eji.1830170527","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T00:40:13Z","timestamp":1172709613000},"page":"739-741","source":"Crossref","is-referenced-by-count":68,"title":["Monoclonal anti\u2010allotype antibody towards BALB\/c IgM Analysis of specificity and site of a V\u2010C crossover in recombinant strain BALB\u2010Igh\u2010V<sup>a<\/sup>\/Igh\u2010C<sup>b<\/sup>"],"prefix":"10.1002","volume":"17","author":[{"given":"Ralph","family":"Sch\u00fcppel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joachim","family":"Wilke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eberhardt","family":"Weiler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","volume-title":"Handbook of Experimental Immunology","author":"Parsons M.","year":"1986"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.73.4.1305"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/265447a0"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00343718"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01844009"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60127-3"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"873","DOI":"10.4049\/jimmunol.127.3.873","volume":"127","author":"Kung J. 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