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The maintenance of DNA synthesis in such cultures was found to provide a sensitive assay for revealing the factors that interact with cycling B cells. Whereas several activities \u2010 namely, interleukin 4, anti\u2010Bp50 and a low molecular weight B cell growth factor \u2010 were, by themselves, capable of prolonging DNA synthesis over a further day or so, no single factor was capable of sustaining the replication cycle out to day 6 of culture. By contrast, certain combinations of activities displayed significant synergy in the restimulation assay. The most striking observed was that between interleukin 4 and anti\u2010Bp50 where, by day 6, their combined effect on maintaining DNA synthesis in 3\u2010day stimulated cells was the same as having kept phorbol dibutyrate and ionomycin in the culture system. The implications of these findings are discussed.<\/jats:p>","DOI":"10.1002\/eji.1830171026","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T00:05:01Z","timestamp":1172707501000},"page":"1535-1538","source":"Crossref","is-referenced-by-count":46,"title":["Synergistic interaction between interleukin 4 and anti\u2010Bp50 (CDw40) revealed in a novel B cell restimulation assay"],"prefix":"10.1002","volume":"17","author":[{"given":"John","family":"Gordon","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle J.","family":"Millsum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Graeme R.","family":"Guy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey A.","family":"Ledbetter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830150216"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.162.4.1319"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/324070a0"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.22.7681"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.165.6.1459"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830161225"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830160908"},{"key":"e_1_2_1_9_2","doi-asserted-by":"crossref","first-page":"98","DOI":"10.4049\/jimmunol.138.1.98","volume":"138","author":"Pezzuto A.","year":"1987","journal-title":"J. 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