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We observed a requirement for N\u2010linked glycosylation in the proper assembly and surface expression of HLA\u2010B locus products in particular, although considerable variation was seen within the allelic series of the HLA\u2010A and B loci. We conclude that the single N\u2010linked glycan can contribute in a major way to that conformation of the heavy (H) chain which is competent to associate with the light chain \u03b2<jats:sub>2<\/jats:sub>\u2010microglobulin, and that the presence, rather than the type, of carbohydrate chain is important in this respect.<\/jats:p><jats:p>The association of human class IH chains with \u03b2<jats:sub>2<\/jats:sub>\u2010microglobulin shows biphasic kinetics, where an initially rapid phase is followed by a prolonged period during which no further association can be measured. It appears that HLA\u2010C H chains are initially synthesized in amounts similar to HLA\u2010A and B H chains, but associate inefficiently with \u03b2<jats:sub>2<\/jats:sub>\u2010microglobulin, resulting in low expression of HLA\u2010C at the cell surface. The individual stages of assembly and maturation of class I antigens including the transfer from Golgi to cell surface were found to display characteristic allelic variation.<\/jats:p>","DOI":"10.1002\/eji.1830180522","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T05:37:56Z","timestamp":1172727476000},"page":"801-810","source":"Crossref","is-referenced-by-count":184,"title":["Allele and locus\u2010specific differences in cell surface expression and the association of HLA class I heavy chain with \u03b2<sub>2<\/sub>\u2010microglobulin: differential effects of inhibition of glycosylation on class I subunit association"],"prefix":"10.1002","volume":"18","author":[{"given":"Jacques J.","family":"Neefjes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidde L.","family":"Ploegh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,11,22]]},"reference":[{"key":"e_1_2_1_2_2","volume-title":"Natural history of the major histocompatibility complex","author":"Klein J.","year":"1986"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-550870-4.50007-2"},{"key":"e_1_2_1_4_2","doi-asserted-by":"crossref","first-page":"270","DOI":"10.4049\/jimmunol.126.1.270","volume":"126","author":"Ploegh H. 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