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The direct precursors of these \u201cdouble\u2010positive\u201d thymocytes are cycling cortical blast cells of the CD4<jats:sup>\u2212<\/jats:sup>8<jats:sup>+<\/jats:sup> phenotype. Using a new monoclonal antibody to a constant determinant of the rat TcR\u03b1\/\u03b2, it is shown here that (a) about 50% of these CD8 \u201csingle\u2010positive\u201d committed precursor cells already express the TcR\u03b1\/\u03b2, though at very low levels, (b) during short\u2010term suspension culture in medium supplemented only with fetal calf serum they not only acquire CD4 but also TcR\u03b1\/\u03b2 levels characteristic of CD4,8 \u201cdouble\u2010positive\u201d thymocytes, and (c) cross\u2010linking of the TcR during culture inhibits the acquisition of the CD4 antigen in the majority of these cells.<\/jats:p>","DOI":"10.1002\/eji.1830181234","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T01:04:15Z","timestamp":1172711055000},"page":"2089-2092","source":"Crossref","is-referenced-by-count":25,"title":["Cross\u2010linking of the T cell antigen receptor interferes with the generation of CD4<sup>+<\/sup>8<sup>+<\/sup> thymocytes from their immediate CD4<sup>\u2212<\/sup>8<sup>+<\/sup> precursors"],"prefix":"10.1002","volume":"18","author":[{"given":"Thomas","family":"H\u00fcnig","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1984.tb01118.x"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.4049\/jimmunol.133.3.1117","volume":"133","author":"Kisielow P.","year":"1984","journal-title":"J. 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