{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T03:43:52Z","timestamp":1770695032388,"version":"3.49.0"},"reference-count":27,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,11,17]],"date-time":"2005-11-17T00:00:00Z","timestamp":1132185600000},"content-version":"vor","delay-in-days":5709,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1990,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Stimulation of murine thymocytes with phorbol ester or calcium ionophore for 18\u201324 h resulted in 70%\u201380% fragmentation of DNA into 180\u2013200\u2010bp multiples, followed by cell death. Experiments with fractionated subpopulations by panning or flow cytometry revealed that DNA fragmentation was selectively observed in CD4<jats:sup>+<\/jats:sup>CD8<jats:sup>+<\/jats:sup> cells and in a portion of CD4<jats:sup>\u2212<\/jats:sup>CD8<jats:sup>+<\/jats:sup> cells. To investigate whether DNA cleavage is also inducible via antigen\u2010specific receptors, thymocytes were incubated in wells precoated with anti\u2010CD3 antibody. An approximately 20% increase of DNA fragmentation was constantly observed when unseparated thymocytes were stimulated with anti\u2010CD3 antibody. In this anti\u2010CD3\u2010induced DNA degradation, CD4<jats:sup>+<\/jats:sup>CD8<jats:sup>+<\/jats:sup> cells are probably the target cells, since (a) fetal thymocytes at day 18 of gestation were found vulnerable to anti\u2010CD3\u2010induced DNA cleavage and (b) flow cytometry analysis of viable cells recovered after cultivation in the anti\u2010CD3\u2010coated wells revealed that CD4<jats:sup>+<\/jats:sup>CD8<jats:sup>+<\/jats:sup> cells were preferentially decreased. Further experiments with purified CD4<jats:sup>+<\/jats:sup>CD8<jats:sup>+<\/jats:sup> cells, however, could not define a clear\u2010cut increase of DNA fragmentation when isolated CD4<jats:sup>+<\/jats:sup>CD8<jats:sup>+<\/jats:sup> cells were stimulated with anti\u2010CD3 antibody. Addition of interleukin (IL) 1, IL 2, IL 3, IL 4 or interferon\u2010\u03b3 to the CD4<jats:sup>+<\/jats:sup>CD8<jats:sup>+<\/jats:sup> cell cultures failed to yield a DNA cleavage similar to that of unseparated thymocytes.<\/jats:p>","DOI":"10.1002\/eji.1830200411","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T11:45:11Z","timestamp":1172749511000},"page":"779-784","source":"Crossref","is-referenced-by-count":59,"title":["CD4<sup>+<\/sup>CD8<sup>+<\/sup> thymocytes are susceptible to DNA fragmentation induced by phorbol ester, calcium ionophore and anti\u2010CD3 antibody"],"prefix":"10.1002","volume":"20","author":[{"given":"Takushi","family":"Tadakuma","sequence":"first","affiliation":[]},{"given":"Harutoshi","family":"Kizaki","sequence":"additional","affiliation":[]},{"given":"Chikako","family":"Odaka","sequence":"additional","affiliation":[]},{"given":"Ryo","family":"Kubota","sequence":"additional","affiliation":[]},{"given":"Yuzuru","family":"Ishimura","sequence":"additional","affiliation":[]},{"given":"Hideo","family":"Yagita","sequence":"additional","affiliation":[]},{"given":"Ko","family":"Okumura","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(87)90568-X"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/333742a0"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/334620a0"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/332040a0"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/335174a0"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60643-4"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.143.6.1790"},{"key":"e_1_2_1_9_2","doi-asserted-by":"crossref","first-page":"2445","DOI":"10.4049\/jimmunol.131.5.2445","volume":"131","author":"Dialynas D. 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