{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T10:48:40Z","timestamp":1770720520539,"version":"3.49.0"},"reference-count":33,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2005,11,17]],"date-time":"2005-11-17T00:00:00Z","timestamp":1132185600000},"content-version":"vor","delay-in-days":5526,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1990,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A clonal assay was used to study different stimuli involved in the progression of fetal liver B cell precursors to mature B lymphocytes.<\/jats:p><jats:p>In this report we replaced fetal liver heterogenous feeder cells by a recombinant growth factor, interleukin 7 (IL 7), and a clonal stromal cell line, S17. Under those conditions we could clone 1 in 10 B220<jats:sup>+<\/jats:sup> B cell precursors from fetal liver and the cells could differentiate to a mitogen\u2010responsive, immunoglobulinsecreting stage. We found that IL 7 stimulates proliferation of B220<jats:sup>+<\/jats:sup> precursors but is not sufficient to support maturation of those precursors to a stage of mitogen responsiveness. We show further that the cell line S17 does not produce IL 7 at functionally detectable level but provides support for B cell maturation. We conclude that this cell line supplies an exogenous stimulus required by B cell precursors to become mature lymphocytes. We describe therefore two stages in pre\u2010B cell development: (a) IL 7\u2010dependent proliferation and (b) S17\u2010dependent maturation to mitogen reactivity. Further studies demonstrate that S17 has a profound effect on B cells by increasing the clonal efficiency of lipopolysaccharide\u2010responsive cells to nearly 1:1 B cell in the spleen of adult C57BL\/6 mice.<\/jats:p>","DOI":"10.1002\/eji.1830201006","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T11:34:39Z","timestamp":1172748879000},"page":"2183-2189","source":"Crossref","is-referenced-by-count":78,"title":["The influence of S17 stromal cells and interleukin 7 on B cell development"],"prefix":"10.1002","volume":"20","author":[{"given":"Ana","family":"Cumano","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth","family":"Dorshkind","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven","family":"Gillis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher J.","family":"Paige","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60032-2"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1986.tb01504.x"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/307233a0"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/309763a0"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.14.5228"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.23.8153"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/309799a0"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3020690"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.11.3608"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/302711a0"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(87)90709-4"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(87)90708-2"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830180606"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.138.4.1082"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830181117"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.168.4.1511"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830141104"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(82)90349-0"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830180115"},{"key":"e_1_2_1_21_2","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1182\/blood.V64.1.152.152","volume":"64","author":"Myers C. 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