{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T04:40:13Z","timestamp":1773290413463,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2005,12,14]],"date-time":"2005-12-14T00:00:00Z","timestamp":1134518400000},"content-version":"vor","delay-in-days":5522,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1990,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The H\u20102K<jats:sup>b<\/jats:sup>\u2010restricted cytotoxic T lymphocyte (CTL) response directed against ovalbumin (OVA) is specific for a region contained within the sequence OVA<jats:sub>253\u2010276<\/jats:sub>. In this study we have characterized this response by examining the class I\u2010restricted presentation of OVA peptides by the naturally occurring K<jats:sup>b<\/jats:sup> mutant (K<jats:sup>bm<\/jats:sup>) glycoproteins K<jats:sup>bm1<\/jats:sup>, K<jats:sup>bm3<\/jats:sup>, K<jats:sup>bm5<\/jats:sup>, K<jats:sup>bm8<\/jats:sup>, K<jats:sup>bm10<\/jats:sup>, K<jats:sup>bm11<\/jats:sup> and K<jats:sup>bm23<\/jats:sup>. To facilitate this study we derived a series of somatic cell hybrid targets expressing the various K<jats:sup>bm<\/jats:sup> class I molecules. Experiments using bulk OVA\u2010specific CTL from C57BL\/6 mice demonstrated that all the K<jats:sup>bm<\/jats:sup> molecules except for K<jats:sup>bm1<\/jats:sup> and K<jats:sup>bm8<\/jats:sup> could present OVA peptides for effective T cell recognition. Clonal analysis revealed a more complex and relatively diverse pattern of CTL recognition of the K<jats:sup>bm<\/jats:sup>\/peptide combinations. This diversity is unlikely to result from the existence of multiple, independent K<jats:sup>b<\/jats:sup>\u2010restricted T cell determinants within OVA, since all CTL tested were specific for a single region between residues 259 and 273. Examination of the fine specificity of K<jats:sup>bm<\/jats:sup> presentation identified individual changes at residues 77, 80 and 116 which affected T cell recognition. The results imply that these changes do not inhibit peptide binding since some clones could recognize peptide presented by a particular K<jats:sup>bm<\/jats:sup> molecule, while other clones could not. All three residues reside within the peptide\u2010binding cleft of the class I protein and are not expected to directly contact the T cell receptor. Although we did not formally demonstrate that OVA binding by K<jats:sup>bm<\/jats:sup> vs. K<jats:sup>b<\/jats:sup> is quantitatively identical, our results are best explained by postulating that the changes at residues 77, 80 and 116 indirectly affect T cell recognition by altering peptide conformation. Taken together our results suggest that changes within the class I binding site can profoundly modify peptide presentation without significantly inhibiting peptide\u2010class I association.<\/jats:p>","DOI":"10.1002\/eji.1830201111","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T11:30:17Z","timestamp":1172748617000},"page":"2431-2437","source":"Crossref","is-referenced-by-count":52,"title":["The effect of mutations in the MHC class I peptide binding groove on the cytotoxic T lymphocyte recognition of the K<sup>b<\/sup>\u2010restricted ovalbumin determinant"],"prefix":"10.1002","volume":"20","author":[{"given":"Janko","family":"Nikoli\u0107\u2010\u017dugi\u0107","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francis R.","family":"Carbone","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,14]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/329506a0"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/329512a0"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/342692a0"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00621950"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(88)90178-X"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(88)91043-4"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.169.3.603"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.171.2.377"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/344065a0"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/344019a0"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.167.6.1767"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/292547a0"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01561661"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00342194"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.153.5.1198"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.161.1.269"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00376522"},{"key":"e_1_2_1_19_2","doi-asserted-by":"crossref","first-page":"1674","DOI":"10.4049\/jimmunol.143.5.1674","volume":"143","author":"Pullen J. 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Immunol."},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.23.9194"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.168.2.725"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.04.040186.002351"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90716-R"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.1830201111","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.1830201111","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T16:09:43Z","timestamp":1697990983000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.1830201111"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,11]]},"references-count":22,"journal-issue":{"issue":"11","published-print":{"date-parts":[[1990,11]]}},"alternative-id":["10.1002\/eji.1830201111"],"URL":"https:\/\/doi.org\/10.1002\/eji.1830201111","archive":["Portico"],"relation":{},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,11]]}}}