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The optimal lymphokine combination was interleukin 2 throughout, together with interferon\u2010\u03b3 during the first 6 days and interleukin 6 during the last 2 days of culture. Under these conditions half of all CD4<jats:sup>\u2212<\/jats:sup>CD8<jats:sup>+<\/jats:sup> T cells became CTL clones. The CTL were CD4<jats:sup>\u2212<\/jats:sup>CD8<jats:sup>+<\/jats:sup>CD3<jats:sup>+<\/jats:sup> TcR \u03b1\/\u03b2<jats:sup>+<\/jats:sup> and were derived from CD4<jats:sup>\u2212<\/jats:sup>CD8<jats:sup>+<\/jats:sup> Pgp\u20101<jats:sup>\u2212<\/jats:sup> precursors.<\/jats:p>","DOI":"10.1002\/eji.1830210432","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T12:38:41Z","timestamp":1172752721000},"page":"1069-1072","source":"Crossref","is-referenced-by-count":22,"title":["Lymphokine requirements for the development of specific cytotoxic T cells from single precursors"],"prefix":"10.1002","volume":"21","author":[{"given":"Lesley A.","family":"Rogers","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Albert","family":"Zlotnik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ken","family":"Shortman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,22]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"1210","DOI":"10.4049\/jimmunol.143.4.1210","volume":"143","author":"Maraskovsky E.","year":"1989","journal-title":"J. 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