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The expression and functional activity of VLA\u20104 has been studied in different maturation and activation stages of B cells from several cellular compartments. Resident B lymphocytes of different lymphoid organs were almost negative for VLA\u20104 as detected by both immunoperoxidase staining and flow cytometry analysis. However, a high expression of both chains of this heterodimer was observed when tonsillar B cells were activated <jats:italic>in vitro<\/jats:italic> with different stimuli, such as phorbol esters or <jats:italic>Staphylococcus aureus<\/jats:italic> Cowan I (SAC). Both nonactivated and <jats:italic>in vitro<\/jats:italic> activated B cells from peripheral blood constitutively expressed high levels of this surface antigen. The induced expression of VLA\u20104 after activation of tonsillar B lymphocytes was accompanied by the acquisition of the capacity to bind to a 38\u2010kDa proteolytic fragment, containing the connecting segment I domain, of FN. Interestingly, nonactivated peripheral blood B cells were unable to attach to this FN fragment, in spite of their constitutive expression of VLA\u20104, and only acquired this functional capacity after cell activation with phorbol esters and SAC. This FN\u2010binding acquisition was not affected by preincubation with inhibitors of protein and RNA synthesis. These results underline that the FN\u2010binding activity of VLA\u20104 is dependent on processes affecting cellular activation as described for other members of the integrin family. By contrast, VLA\u20104\u2010mediated homotypic aggregation of peripheral blood B cells could be triggered by anti\u2010\u03b14 monoclonal antibodies independently of the cell activation state.<\/jats:p>","DOI":"10.1002\/eji.1830211021","type":"journal-article","created":{"date-parts":[[2007,3,9]],"date-time":"2007-03-09T09:41:43Z","timestamp":1173433303000},"page":"2437-2445","source":"Crossref","is-referenced-by-count":30,"title":["Differential expression of VLA\u20104 integrin by resident and peripheral blood B lymphocytes. Acquisition of functionally active \u03b14\u03b21\u2010fibronectin receptors upon B cell activation"],"prefix":"10.1002","volume":"21","author":[{"given":"Antonio A.","family":"Postigo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael","family":"Pulido","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel R.","family":"Campanero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agust\u00edn","family":"Acevedo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angeles","family":"Garc\u00eda\u2010Pardo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angel L.","family":"Corbi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco","family":"Sanchez\u2010Madrid","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuel O.","family":"De Landazuri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,22]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(89)90061-3"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60645-8"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/304030a0"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.170.1.123"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/342078a0"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"2995","DOI":"10.4049\/jimmunol.134.5.2995","volume":"134","author":"Kansas G. 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