{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T19:50:44Z","timestamp":1762113044745},"reference-count":32,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2005,11,22]],"date-time":"2005-11-22T00:00:00Z","timestamp":1132617600000},"content-version":"vor","delay-in-days":5166,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1991,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Rat peritoneal polymorphonuclear leukocytes (PMN) elicited with oyster glycogen contain a Ca<jats:sup>2+<\/jats:sup>\u2010independent nitric oxide (NO) synthase which is induced <jats:italic>in vivo<\/jats:italic> in a time\u2010dependent manner. When washed PMN containing low levels of enzyme activity were cultured <jats:italic>ex vivo<\/jats:italic> further expression of NO synthase was observed. This was inhibited by cycloheximide indicating that <jats:italic>de novo<\/jats:italic> synthesis of the enzyme occurred during the <jats:italic>ex vivo<\/jats:italic> incubation. Enzyme activity was enhanced by interferon (IFN)\u2010\u03b3, but not by tumor necrosis factor (TNF)\u2010\u03b1 when added <jats:italic>ex vivo.<\/jats:italic> However, IFN\u2010\u03b3 and TNF\u2010\u03b1 synergized to increase further the expression of NO synthase.<\/jats:p><jats:p>Treatment of rats with dexamethasone inhibited the induction of NO synthase in elicited PMN. This treatment reduced the accumulation of PMN by approximately 30%, without affecting cell viability. Dexamethasone also inhibited the induction of the NO synthase <jats:italic>ex vivo<\/jats:italic> in a concentration\u2010dependent manner. Furthermore, the enhanced enzyme activity following treatment of PMN with cytokines was also inhibited by dexamethasone. Once induced, dexamethasone did not affect enzyme activity. These data indicate that PMN elicited in the rat peritoneum with oyster glycogen express an NO synthase <jats:italic>in vivo<\/jats:italic> and <jats:italic>ex vivo.<\/jats:italic> The induction of the enzyme can be further stimulated <jats:italic>ex vivo<\/jats:italic> with IFN\u2010\u03b3 and TNF\u2010\u03b1 and inhibited by dexamethasone. The inhibition of the induction of NO synthase in the PMN by dexamethasone may contribute to the anti\u2010inflammatory activity of this and other glucocorticoids.<\/jats:p>","DOI":"10.1002\/eji.1830211032","type":"journal-article","created":{"date-parts":[[2007,3,9]],"date-time":"2007-03-09T10:35:58Z","timestamp":1173436558000},"page":"2523-2527","source":"Crossref","is-referenced-by-count":117,"title":["Induction of nitric oxide synthase in rat peritoneal neutrophils and its inhibition by dexamethasone"],"prefix":"10.1002","volume":"21","author":[{"given":"Th\u00e9r\u00e8se B.","family":"McCall","sequence":"first","affiliation":[]},{"given":"Richard M. 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