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Expression of this receptor is restricted to B lymphocytes, immature thymocytes, and certain epithelial cells. In the present investigation, we describe the presence of a seemingly novel EBV receptor which is phenotypically distinct from CR2. Among various leukemic T cells studied, one, HSB\u20102, demonstrates no reactivity to several anti\u2010CR2 antibodies, yet it reacts strongly with EBV as detected by incubation with biotin\u2010conjugated virus and streptavidin\u2010phycoerythrin. The virus binding is specific as demonstrated by blocking with anti\u2010EBV antibodies and with non\u2010conjugated virus. Aggregated C3 also binds HSB\u20102 and is capable of partially inhibiting EBV binding. The absence of CR2 on HSB\u20102 is further supported by the lack of expression of specific mRNA, assessed by Northern blotting analysis and polymerase chain reaction. Viral internalization and infection is demonstrated with electron microscopy, with detection of EBV\u2010DNA by Southern blotting, and with detection of EBNA\u20101 transcripts by the polymerase chain reaction. Even though HSB\u20102 does not express CR2, it nevertheless displays transcripts which have some homology to a CR2 cDNA probe under low stringency hybridization conditions. This probe encompasses approximately the N\u2010terminal half of CR2 which includes the EBV\u2010binding epitope(s). The HSB\u20102 message is 5.2 kb, a size distinct from the 4.7\u2010kb message of B cell CR2s. In contrast, the 5.2\u2010kb message in not seen, under similar hybridization conditions, with a probe comprising the C\u2010terminal half of CR2. Collectively, the data indicate that a receptor molecule having distinct phenotypic characteristics from the known CR2 protein on B cells is utilized by EBV to target human T lymphocytes.<\/jats:p>","DOI":"10.1002\/eji.1830220504","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T17:34:50Z","timestamp":1172770490000},"page":"1123-1131","source":"Crossref","is-referenced-by-count":16,"title":["Interaction between Epstein\u2010Barr virus and a T cell line (HSB\u20102) via a receptor phenotypically distinct from complement receptor type 2"],"prefix":"10.1002","volume":"22","author":[{"given":"Joseph A.","family":"Hedrick","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Debbie","family":"Watry","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Speiser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"O'Donnell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John D.","family":"Lambris","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Constantine D.","family":"Tsoukas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,7]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.31.100177.002225"},{"key":"e_1_2_1_3_2","first-page":"231","volume":"60","author":"Purtilo D. 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