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Class I heterodimers that have failed to bind peptide (\u201cempty\u201d class I molecules) dissociate and the class I heavychains are degraded. Internalization, if it precedes breakdown, would be the rate\u2010limiting step.<\/jats:p><jats:p>Radioiodinated peptides (VSV NP8\u2010mer or Sendai NP9\u2010mer) dissociate from the class I molecules in the absence of exogenous peptide or \u03b2<jats:sub>2<\/jats:sub> microglobulin and appear in the medium. Release of the iodinated peptides does notresult in a reduction in the quantity of stable assembled class I molecules. This paradox may be explained by a more rapid off\u2010rate for radioiodinated peptides, when compared with their unlabeled counterparts, which constitute about 99% of the total in our radiolabeled preparations. In the medium the peptide is rapidly modified by serum\u2010 and cell\u2010derived proteases. 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