{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T10:43:21Z","timestamp":1770720201448,"version":"3.49.0"},"reference-count":28,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2005,11,17]],"date-time":"2005-11-17T00:00:00Z","timestamp":1132185600000},"content-version":"vor","delay-in-days":4795,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1992,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The hyaluronic acid (HA)\u2010binding function of CD44 expressed on the cell surface of normal hematopoietic cells has been studied by assaying binding of fluoresceinated hyaluronic acid (FI\u2010HA) and adhesion to immobilized HA. As has been observed previously, normal hematopoietic cells from bone marrow and spleen do not constitutively bind HA. A CD44\u2010specific monoclonal antibody, IRAWB 14, which has been shown to rapidly induce HA binding in some CD44<jats:sup>+<\/jats:sup> cell lines, was used to activate the HA\u2010binding function of CD44 in these normal cells. Only splenic T cells were activated by the IRAWB 14 antibody to bind FI\u2010HA. Upon activation, FI\u2010HA binding correlated with the level of CD44 expression.<\/jats:p><jats:p>Activation of HA binding allowed splenic T cells to adhere to HA immobilized on plastic and to an endothelial cell line in an HA\u2010dependent manner. BALB\/c and AKR\/J splenic T cells differ in their level of CD44 expression, and this correlated with differences in their ability to bind HA upon antibody activation. The minor subpopulation of MEL\u201014<jats:sup>\u2212<\/jats:sup> T cells were among the brightest Fl\u2010HA\u2010staining cells.<\/jats:p><jats:p>We propose, on the basis of these and other results, that there are three states of CD44 function with respect to HA binding: (a) a <jats:italic>non\u2010activatable<\/jats:italic>, resting state, which cannot be rapidly activated to bind HA, as seen in most hematopoietic cells; (b) an <jats:italic>activatable<\/jats:italic> state, which can be rapidly converted to HA\u2010binding function, in this case by the IRAWB 14 antibody, illustrated by T cells as shown here; and (c) a <jats:italic>constitutively active<\/jats:italic> state, which can bind HA without antibody activation, seen in some cell lines.<\/jats:p>","DOI":"10.1002\/eji.1830221036","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T13:13:36Z","timestamp":1172754816000},"page":"2719-2723","source":"Crossref","is-referenced-by-count":98,"title":["CD44 can be activated to function as an hyaluronic acid receptor in normal murine T cells"],"prefix":"10.1002","volume":"22","author":[{"given":"Jayne","family":"Lesley","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Hyman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.172.1.69"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90694-A"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(90)90085-O"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1991.tb07955.x"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.175.1.257"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"1422","DOI":"10.4049\/jimmunol.146.5.1422","volume":"146","author":"Murakami S.","year":"1991","journal-title":"J. Immunol."},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00216699"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(85)90237-0"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(91)90431-S"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(88)90274-2"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(90)90007-E"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830190202"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/304030a0"},{"key":"e_1_2_1_15_2","doi-asserted-by":"crossref","first-page":"3618","DOI":"10.4049\/jimmunol.145.11.3618","volume":"145","author":"Murakami S.","year":"1990","journal-title":"J. Immunol."},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(90)90112-M"},{"key":"e_1_2_1_17_2","doi-asserted-by":"crossref","first-page":"3120","DOI":"10.4049\/jimmunol.138.10.3120","volume":"138","author":"Budd R. C.","year":"1987","journal-title":"J. Immunol."},{"key":"e_1_2_1_18_2","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1084\/jem.175.4.895","volume":"175","author":"Rodrigues M.","year":"1992","journal-title":"J. Exp. Med."},{"key":"e_1_2_1_19_2","doi-asserted-by":"crossref","first-page":"2348","DOI":"10.4049\/jimmunol.148.8.2348","volume":"148","author":"Mobley J. L.","year":"1992","journal-title":"J. Immunol."},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.140.5.1401"},{"key":"e_1_2_1_21_2","doi-asserted-by":"crossref","first-page":"140","DOI":"10.4049\/jimmunol.145.1.140","volume":"145","author":"Oppenheimer\u2010Marks N.","year":"1990","journal-title":"J. Immunol."},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.174.3.547"},{"key":"e_1_2_1_23_2","doi-asserted-by":"crossref","first-page":"324","DOI":"10.4049\/jimmunol.148.2.324","volume":"148","author":"Bradley L. M.","year":"1992","journal-title":"J. Immunol."},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.09.040191.000331"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.1.12.883"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.107.5.1893"},{"key":"e_1_2_1_27_2","doi-asserted-by":"crossref","first-page":"685","DOI":"10.4049\/jimmunol.146.2.685","volume":"146","author":"Graham I.","year":"1991","journal-title":"J. Immunol."},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/350068a0"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/349691a0"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.1830221036","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.1830221036","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T10:20:55Z","timestamp":1698056455000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.1830221036"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,10]]},"references-count":28,"journal-issue":{"issue":"10","published-print":{"date-parts":[[1992,10]]}},"alternative-id":["10.1002\/eji.1830221036"],"URL":"https:\/\/doi.org\/10.1002\/eji.1830221036","archive":["Portico"],"relation":{},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,10]]}}}