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TcR \u03b1\u2010chain usage was restricted with three predominant V\u03b1 (V\u03b1 12.1, 14.1, 22) and two predominant J\u03b1 segments. \u03b2\u2010chain variable\u2010region usage was also conserved, with V\u03b27 being used by five clones despite contributing less than 2% of peripheral blood lymphocyte V\u03b2 sequences of one individual studied. The TcR \u03b2\u2010chain junctional region was highly conserved even between CTL clones from unrelated individuals, with a negatively charged amino acid, contributed to by N\u2010region addition, encoded at position 97 in all but two clones. This study shows that peptide\u2010specific HLA B27\u2010restricted CTL following influenza virus infection use very similar TcR and, when considered with previous studies, suggests a pattern of TcR conservation for major histocompatibility complex class I\u2010restricted responses. No difference in TcR usage was detected between one healthy donor and two with HLA B27\u2010associated arthritis.<\/jats:p>","DOI":"10.1002\/eji.1830230702","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T19:03:53Z","timestamp":1172775833000},"page":"1417-1421","source":"Crossref","is-referenced-by-count":58,"title":["Conservation of T cell receptor usage by HLA B27\u2010restricted influenza\u2010specific cytotoxic T lymphocytes suggests a general pattern for antigen\u2010specific major histocompatibility complex class I\u2010restricted responses"],"prefix":"10.1002","volume":"23","author":[{"given":"Paul","family":"Bowness","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul A. 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