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We have isolated full\u2010length cDNA clones from a \u03bbgtl0 library, prepared from phorbol 12\u2010myristate 13\u2010acetate\u2010differentiated HL60 cells by screening with an endoglin\u2010specific cDNA probe from endothelial cells. Sequencing of the largest clone (3073 bp), revealed that the leader sequence contains 25 residues and that the 586 amino acids of the extracellular and transmembrane domains were identical to those described for endothelial endoglin. However, the cytoplasmic tail encoded by this cDNA clone contains only 14 amino acids as opposed to the 47 residues previously reported, suggesting the existence of two alternative endoglin variants. The expression of these isoforms was demonstrated by polymerase chain reaction analyses on endothelial cells, myelomonocytic cell lines HL\u201060 and U\u2010937, and placenta. Independent cDN A constructs corresponding to both forms were transfected into mouse fibroblasts leading to the expression of two distinct endoglin molecules. Both forms were shown to bind TGF\u2010\u03b21 and, when overexpressed in transfected mouse fibroblasts, to form disulfide\u2010linked homodimers, indicating that the cysteine residues present in the extracellular domain are responsible for the dimerization.<\/jats:p>","DOI":"10.1002\/eji.1830230943","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T19:24:01Z","timestamp":1172777041000},"page":"2340-2345","source":"Crossref","is-referenced-by-count":166,"title":["Identification and expression of two forms of the human transforming growth factor\u2010\u03b2\u2010binding protein endoglin with distinct cytoplasmic regions"],"prefix":"10.1002","volume":"23","author":[{"given":"Teresa","family":"Bell\u00f3n","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angel","family":"Corbi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro","family":"Lastres","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmela","family":"Cal\u00e9s","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Cebri\u00e1n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonia","family":"Vera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sela","family":"Cheifetz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joan","family":"Massague","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle","family":"Letarte","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmelo","family":"Bernab\u00e9u","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,11]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.141.6.1925"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830220216"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.1992.tb05848.x"},{"key":"e_1_2_1_5_2","first-page":"841","volume":"5","author":"B\u00fchring H. 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