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We have isolated a novel set of J\u03b1 probes which, in combination with a few restriction enzyme digests, enable a reliable, simple and nearly complete analysis and location of any rearrangement at the J\u03b1 locus by conventional Southern blotting. The probes were used to analyze TcR \u03b1 gene rearrangements in T cell hybridomas derived from an <jats:italic>in vitro<\/jats:italic> culture system that supports T cell differentiation of bone marrow cells. Our results indicate that J\u03b1 genes are unequally accessible for rearrangement and two hot spots for rearrangement could be demonstrated. In addition, only a restricted set of J\u03b1 genes was rearranged in each culture indicating that the slightly variable composition of factors can influence the recombinatorial accessibility of J\u03b1 genes. The hot spots for rearrangement were not only limited to T cells differentiating <jats:italic>in vitro<\/jats:italic> but could also be demonstrated among functional T cell clones based on the published sequence information from isolated TcR \u03b1 gene rearrangements. The demonstration and the location of the hot spots for rearrangement in the T cell differentiation culture system opens up the possibility to study factors and mechanisms that regulate recombinatorial accessibility of TcR \u03b1 genes.<\/jats:p>","DOI":"10.1002\/eji.1830240117","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T19:23:51Z","timestamp":1172777031000},"page":"107-115","source":"Crossref","is-referenced-by-count":12,"title":["Evidence for recombinatorial hot spots at the T cell receptor J\u03b1 locus"],"prefix":"10.1002","volume":"24","author":[{"given":"Marja","family":"Rytk\u00f6nen","sequence":"first","affiliation":[]},{"given":"Julia L.","family":"Hurwitz","sequence":"additional","affiliation":[]},{"given":"Kirsti","family":"Tolonen","sequence":"additional","affiliation":[]},{"given":"Jukka","family":"Pelkonen","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,11,23]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"722","volume":"330","author":"Hayday A. 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