{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T06:18:39Z","timestamp":1770704319735,"version":"3.49.0"},"reference-count":31,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2005,11,23]],"date-time":"2005-11-23T00:00:00Z","timestamp":1132704000000},"content-version":"vor","delay-in-days":4101,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1994,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human melanomas are infiltrated by tumor\u2010reactive T lymphocytes. However, the ability of these cells to elicit a specific anti\u2010tumor response <jats:italic>in vivo<\/jats:italic> remains to be established. Because lymphokine production is critical for T cell functions, we have analyzed the capacity of melanoma\u2010specific tumor\u2010infiltrating lymphocyte (TIL) clones to produce major lymphokines: interleukin\u20102 (IL\u20102), interferon\u2010\u03b3 (IFN\u2010\u03b3) and interleukin\u20104 (IL\u20104), as well as tumor necrosis factor (TNF), in response to direct antigen presentation by autologous and allogeneic tumor cells. We report here that, upon stimulation by autologous melanoma cells, all TIL clones secreted TNF but only a few of them produced significant amounts of IL\u20102, IL\u20104 or IFN\u2010\u03b3. Nonetheless, all these clones consistently produced two or three of these last lymphokines upon stimulation with phorbol myristate acetate and calcium ionophore, as well as IL\u20102 upon CD3 stimulation, showing the existence of three lymphokine profiles among them: Th1, Th0 and a profile characterized by IL\u20102 and IL\u20104, but not IFN\u2010\u03b3 secretion. Stimulation of TIL clones by allogeneic melanoma lines sharing the appropriate HLA\u2010peptide complexes revealed that defective IL\u20102 production seemed to be a constant feature for some clones, while it was, for other clones, dependent on the antigen\u2010presenting tumor cells. For this last type of clone, we further showed that defective IL\u20102 induction resulted from an LFA\u20103 defect of some melanoma cells or from distinct yet undefined defects of other melanoma lines. Our data suggest that defective lymphokine secretion may be an essential component of the <jats:italic>in vivo<\/jats:italic> failure of melanoma\u2010reactive TIL to control tumor development. Interestingly both CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> TIL clones from one patient were fully activated by the autologous melanoma cells <jats:italic>in vitro<\/jats:italic>, supporting a potential role of such TIL in spontaneous or induced tumor rejection.<\/jats:p>","DOI":"10.1002\/eji.1830240905","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T23:14:53Z","timestamp":1172790893000},"page":"1966-1973","source":"Crossref","is-referenced-by-count":39,"title":["Defective lymphokine production by most CD8<sup>+<\/sup> and CD4<sup>+<\/sup> tumor\u2010specific T cell clones derived from human melanoma\u2010infiltrating lymphocytes in response to autologous tumor cells <i>in vitro<\/i>"],"prefix":"10.1002","volume":"24","author":[{"given":"Yannick","family":"Guilloux","sequence":"first","affiliation":[]},{"given":"Christophe","family":"Viret","sequence":"additional","affiliation":[]},{"given":"Nadine","family":"Gervois","sequence":"additional","affiliation":[]},{"given":"Eric Le","family":"Dr\u00e9an","sequence":"additional","affiliation":[]},{"given":"Marie\u2010Christine","family":"Pandolfino","sequence":"additional","affiliation":[]},{"given":"Elisabeth","family":"Diez","sequence":"additional","affiliation":[]},{"given":"Francine","family":"Jotereau","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,11,23]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"189","volume":"138","author":"Muul L. M.","year":"1987","journal-title":"J. Immunol."},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830200417"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830220719"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"3714","DOI":"10.4049\/jimmunol.142.10.3714","volume":"142","author":"Topalian S. L.","year":"1989","journal-title":"J. Immunol."},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.168.4.1419"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"1692","DOI":"10.4049\/jimmunol.146.5.1692","volume":"146","author":"Crowley N. J.","year":"1991","journal-title":"J. Immunol."},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"638","DOI":"10.4049\/jimmunol.148.2.638","volume":"148","author":"Kawakami Y.","year":"1992","journal-title":"J. Immunol."},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830230123"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1840703"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.176.5.1453"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.173.3.647"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.144.12.4898"},{"key":"e_1_2_1_14_2","doi-asserted-by":"crossref","first-page":"3674","DOI":"10.4049\/jimmunol.146.10.3674","volume":"146","author":"Schwartzentruber D. J.","year":"1991","journal-title":"J. Immunol."},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01756190"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12358425"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910570111"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00185107"},{"key":"e_1_2_1_19_2","doi-asserted-by":"crossref","first-page":"672","DOI":"10.4049\/jimmunol.148.3.672","volume":"148","author":"Deckert M.","year":"1992","journal-title":"J. Immunol."},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.07.040189.001045"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1681588"},{"key":"e_1_2_1_22_2","doi-asserted-by":"crossref","first-page":"2942","DOI":"10.4049\/jimmunol.147.9.2942","volume":"147","author":"Wierenga E. A.","year":"1991","journal-title":"J. Immunol."},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.141.3.849"},{"key":"e_1_2_1_24_2","doi-asserted-by":"crossref","first-page":"518","DOI":"10.4049\/jimmunol.143.2.518","volume":"143","author":"Firestein G. S.","year":"1989","journal-title":"J. Immunol."},{"key":"e_1_2_1_25_2","doi-asserted-by":"crossref","first-page":"1629","DOI":"10.4049\/jimmunol.144.5.1629","volume":"144","author":"Street N. E.","year":"1990","journal-title":"J. Immunol."},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.170.6.2081"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.178.5.1597"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.14.6586"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/5.12.1501"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(93)90200-5"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.178.2.489"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19851101)56:9<2287::AID-CNCR2820560924>3.0.CO;2-Y"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.1830240905","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.1830240905","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T13:16:01Z","timestamp":1698153361000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.1830240905"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,9]]},"references-count":31,"journal-issue":{"issue":"9","published-print":{"date-parts":[[1994,9]]}},"alternative-id":["10.1002\/eji.1830240905"],"URL":"https:\/\/doi.org\/10.1002\/eji.1830240905","archive":["Portico"],"relation":{},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,9]]}}}