{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T10:25:53Z","timestamp":1770719153592,"version":"3.49.0"},"reference-count":22,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2005,12,1]],"date-time":"2005-12-01T00:00:00Z","timestamp":1133395200000},"content-version":"vor","delay-in-days":4018,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1994,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>APO\u20101\/Fas (CD95) is a transmembrane receptor that transduces apoptotic signals within various cells including T and B cells. The APO\u20101 gene was found to be defective in <jats:italic>lpr<\/jats:italic> mice. In these mice insertion of a retrotransposon gives rise to transcription of an abnormal mRNA and only a fraction of wild\u2010type APO\u20101 mRNA. It is not clear if <jats:italic>lpr<\/jats:italic> mice still express wild\u2010type APO\u20101 protein. To address this question, we prepared rabbit anti\u2010APO\u20101 antibodies (Ab) with a peptide representing the extracellular sequence corresponding to residues 5\u201023 of APO\u20101. The rabbit Ab reacted with thymocytes from different mouse strains and the extent of binding was correlated with the two known APO\u20101 alleles. In addition, the Ab reacted with mouse cell lines expressing mouse APO\u20101 mRNA but not with human cell lines. Binding of the Ab to MRL and BALB\/c thymocytes was completely blocked by the immunizing peptide. Immunofluorescence analysis of MRL\/<jats:italic>lpr<\/jats:italic> thymocytes showed that they still express APO\u20101 protein at approximately one tenth of the wild\u2010type expression level on their surface. In addition, in <jats:italic>lpr<\/jats:italic> as in wild\u2010type mice we found a decrease of APO\u20101 expression in the more mature thymic compartment. Western blot analysis of whole cell lysates from <jats:italic>lpr<\/jats:italic> and wild\u2010type thymocytes showed that the Ab recognized APO\u20101 in both cell types. Approximately 50% of CD3<jats:sup>+<\/jats:sup> splenocytes and 80% of <jats:italic>in vitro<\/jats:italic> activated CD3<jats:sup>+<\/jats:sup> cells from wild\u2010type mice reacted with the Ab, but to a lower extent than thymocytes. The same differential reactivity was found in <jats:italic>lpr<\/jats:italic> CD3<jats:sup>+<\/jats:sup> splenocytes. <jats:italic>lpr<\/jats:italic> T cells, however, showed a substantially lower level of APO\u20101. Thus, the differential expression of APO\u20101 on thymic versus peripheral <jats:italic>lpr<\/jats:italic> T cells might influence their sensitivity towards APO\u20101\u2010mediated apoptosis.<\/jats:p>","DOI":"10.1002\/eji.1830241231","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T19:52:12Z","timestamp":1172778732000},"page":"3119-3123","source":"Crossref","is-referenced-by-count":48,"title":["The APO\u20101\/Fas (CD95) receptor is expressed in homozygous MRL\/<i>lpr<\/i> mice"],"prefix":"10.1002","volume":"24","author":[{"given":"Sara M.","family":"Mariani","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernd","family":"Matiba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena A.","family":"Armandola","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter H.","family":"Krammer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"279","volume":"6","author":"Krammer P. 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