{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T17:11:26Z","timestamp":1758820286032},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,11,17]],"date-time":"2005-11-17T00:00:00Z","timestamp":1132185600000},"content-version":"vor","delay-in-days":3973,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1995,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The human type two IgG binding receptors (Fc\u03b3RII) are encoded by three genes (Fc\u03b3RIIA, \u2010B and C) resulting in at least six protein isoforms generated by alternative mRNA splicing. Surface expression of Fc\u03b3RII has been shown to be modulated during B cell activation, although data characterizing the isoform(s) expressed are not available. The extracellular as well as the transmembrane domains of various Fc\u03b3;RII are highly homologous. Only the intracellular domains vary between the different Fc\u03b3RII isoforms, suggesting differences in signal transduction. Using reverse transcriptase and polymerase chain reaction of mRNA obtained from resting tonsil B cells, we show that the majority of Fc\u03b3RII mRNA species to be of b2 type, although b1 type and a low level of Fc\u03b3RIIa type are also present. Culturing the cells for 18 h in the presence of 2.5 U\/ml interleukin\u20104 or 10 \u03bcg\/ml affinity\u2010purified anti\u2010IgM F(ab')<jats:sub>2<\/jats:sub> fragments induced a switch in alternative splicing, resulting in a significant increase of Fc\u03b3RIIb1 mRNA expression, while the synthesis of Fc\u03b3RIIb2 mRNA was down\u2010regulated. Stimulation of B cells with 100 ng\/ml phorbol 12\u2010myristate 13\u2010acetate induced similar alteration, although only after 48\u2010h treatment. The accumulation of Fc\u03b3RIIb1 and the reduction of both Fc\u03b3RIIb2 and Fc\u03b3RIIa mRNA in activated cells is accompanied by the enhanced expession of Fc\u03b3RII on the cell surface, representing most probably the Fc\u03b3RIIb1 isoform. Heat\u2010aggregated IgG inhibited the anti\u2010IgM\u2010induced proliferation of resting but not that of activated B cells, suggesting that aggregation of Fc\u03b3RIIb2 constitutively expressed on resting B cells might be responsible for the prevention of inadequate activation of resting B cells.<\/jats:p>","DOI":"10.1002\/eji.1830250143","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T23:14:59Z","timestamp":1172790899000},"page":"262-268","source":"Crossref","is-referenced-by-count":16,"title":["The alternative splicing of human Fc\u03b3RII mRNA is regulated by activation of B cells with mIgM cross\u2010linking, interleukin\u20104, or phorbolester"],"prefix":"10.1002","volume":"25","author":[{"given":"Gabriella","family":"S\u00e1rmay","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zolt\u00e1n","family":"Rozsnyay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u00e1bor","family":"Koncz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alla","family":"Danilkovich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00e1nos","family":"Gergely","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(93)90166-I"},{"key":"e_1_2_1_3_2","first-page":"355","volume":"5","author":"Fridman W. 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