{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T11:06:58Z","timestamp":1770721618824,"version":"3.49.0"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,12,1]],"date-time":"2005-12-01T00:00:00Z","timestamp":1133395200000},"content-version":"vor","delay-in-days":3622,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1996,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Transgenic mice in which mouse interleukin (IL)\u20107 cDNA is expressed under the control of the mouse major histocompatibility complex (MHC) class II (E\u03b1) promoter develop a lymphoproliferative disease characterized by the early polyclonal expansion of T cells followed in many cases by the development of lymphomas of immature B cells. Here, we have analyzed B cell development in these transgenic mice. Phenotypic analysis using monoclonal antibodies to B220, IgM, IgD, <jats:italic>c\u2010kit<\/jats:italic>, IL\u20107 receptor, MHC class II, AA4.1, CD19, CD23, CD25, CD40 and CD43 shows that B lymphopoiesis in the bone marrow is dramatically altered and the number of pro\/pre\u2010B and immature B cells is significantly increased. Interestingly, pro\/pre\u2010B and immature B cells persist in the spleens of adult transgenic mice and are also present in lymph nodes and blood. Cell cycle analysis of lymph node cells shows that subpopulations of developing B cells retain the cell cycle profiles of their bone marrow counterparts. Limiting dilution analysis shows that the number of clonable pre\u2010B cells is significantly increased and that at limiting dilution, growth of transgenic pre\u2010B cells is still dependent on exogenous IL\u20107. Using semiquantitative polymerase chain reaction (PCR) and <jats:italic>in situ<\/jats:italic> hybridization, the level of IL\u20107 transcripts in the spleen was found to decrease between 2 and 4 weeks in control mice with levels in transgenics mice being approximately 50 times greater. These transgenic mice represent an interesting model with which to study the effects of IL\u20107 overexpression in the bonemarrow and raise interesting questions regarding the regulation of B lymphopoiesis in normal mice.<\/jats:p>","DOI":"10.1002\/eji.1830260105","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T01:16:14Z","timestamp":1172798174000},"page":"28-33","source":"Crossref","is-referenced-by-count":46,"title":["Phenotypic and functional analysis of B lymphopoiesis in interleukin\u20107\u2010transgenic mice: expansion of pro\/pre\u2010B cell number and persistence of B lymphocyte development in lymph nodes and spleen"],"prefix":"10.1002","volume":"26","author":[{"given":"Elisabeth","family":"Mertsching","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulf","family":"Grawunder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valerie","family":"Meyer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ton","family":"Rolink","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rhodri","family":"Ceredig","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.181.4.1519"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.178.1.257"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.180.5.1955"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.2.377"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/333571a0"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1002\/j.1460-2075.1991.tb07953.x","volume":"10","author":"Rolink A. A.","year":"1991","journal-title":"EMBO J."},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"2290","DOI":"10.1182\/blood.V81.9.2290.2290","volume":"81","author":"Rolink A.","year":"1993","journal-title":"Blood"},{"key":"e_1_2_1_9_2","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1182\/blood.V79.5.1121.1121","volume":"79","author":"Damia G.","year":"1992","journal-title":"Blood"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/7.3.415"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/7.3.401"},{"key":"e_1_2_1_12_2","doi-asserted-by":"crossref","first-page":"561","DOI":"10.4049\/jimmunol.147.2.561","volume":"147","author":"Morrissey P. J.","year":"1991","journal-title":"J. Immunol."},{"key":"e_1_2_1_13_2","doi-asserted-by":"crossref","first-page":"2409","DOI":"10.4049\/jimmunol.151.5.2409","volume":"151","author":"Fraser C. C.","year":"1993","journal-title":"J. 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