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This cell line was generated from mouse fetal skin and was previously shown to have the characteristics of early DC precursors. Both FSDC and bone marrow\u2010derived DC (BM\u2010DC) were more effective in the pinocytosis of FITC\u2010conjugated ovalbumin (FITC\u2010OVA) and dextran (FITC\u2010DX) than B cells or macrophages. Pretreatment of FSDC with granulocyte\/macrophage colony\u2010stimulating factor (GM\u2010CSF) \u00b1 interleukin (IL)\u20104 enhanced the pinocytic uptake of FITC\u2010OVA and FITC\u2010DX, but did not induce antigen\u2010presenting capacity. In contrast, untreated FSDC or FSDC pre\u2010incubated with GM\u2010CSF \u00b1 IL\u20104 suppressed T cell responses. Treatment of FSDC with IFN\u2010\u03b3 reduced pinocytosis but increased the expression of MHC and co\u2010stimulatory\/adhesion molecules and promoted efficient presentation of OVA protein or peptide to the specific DO11.10 T cell hybridoma or to naive CD4<jats:sup>+<\/jats:sup> T cells from DO11.10 TCR\u2010transgenic mice. The results suggest that antigen uptake and antigen presentation in DC are regulated by different cytokine signals provided by the surrounding tissue.<\/jats:p>","DOI":"10.1002\/eji.1830260313","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T01:50:12Z","timestamp":1172800212000},"page":"586-594","source":"Crossref","is-referenced-by-count":83,"title":["Different cytokines regulate antigen uptake and presentation of a precursor dendritic cell line"],"prefix":"10.1002","volume":"26","author":[{"given":"Manfred B.","family":"Lutz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline U.","family":"A\u00dfmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giampiero","family":"Girolomoni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Ricciardi\u2010Castagnoli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/282324a0"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.7.3038"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.179.1.203"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(92)90199-4"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.09.040191.001415"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.175.5.1157"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.180.1.83"},{"key":"e_1_2_1_9_2","first-page":"487","volume":"82","author":"O'doherty U.","year":"1994","journal-title":"Immunology"},{"key":"e_1_2_1_10_2","first-page":"324","volume-title":"Epidermal Langerhans Cells","author":"Schuler G.","year":"1991"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00200527"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(95)80118-9"},{"key":"e_1_2_1_13_2","first-page":"395","volume":"81","author":"Cumberbatch M.","year":"1994","journal-title":"Immunology"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.172.2.631"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.170.4.1285"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.166.6.1654"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.181.6.2237"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.178.2.509"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.139.2.380"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830160405"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12275566"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.17.8342"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.182.2.389"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.169.3.1169"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.166.1.182"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.169.4.1255"},{"key":"e_1_2_1_27_2","doi-asserted-by":"crossref","first-page":"6742","DOI":"10.4049\/jimmunol.151.12.6742","volume":"151","author":"Levin D.","year":"1993","journal-title":"J. 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