{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T15:05:24Z","timestamp":1778943924895,"version":"3.51.4"},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,12,1]],"date-time":"2005-12-01T00:00:00Z","timestamp":1133395200000},"content-version":"vor","delay-in-days":3531,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1996,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have studied the expression of the novel anti\u2010apoptotic protein bcl\u2010x during mouse B cell differentiation and activation. We find that bcl\u2010x is expressed throughout all stages of B cell differentiation in the bone marrow, and is only down\u2010regulated in mature (sIgD<jats:sup>+<\/jats:sup>) B cells. Immature peripheral B cells express low levels of bcl\u2010x even in adult animals, whereas mature resting B cells do not. Mature B cells re\u2010express the protein following activation, achieving maximal levels after 36\u201348 h. The highest levels of bcl\u2010x are observed with potent comitogenic stimuli (such as anti\u2010CD40 + anti\u2010Ig): B cells first express bcl\u2010x in the G1 phase of the cell cycle and contain maximal levels in S phase. In addition, B cells from CBA\/N mice, which do not proliferate when stimulated with anti\u2010Ig, anti\u2010CD40, or both, exhibited only low levels of the protein following culture with these stimuli. To investigate the functional significance of bcl\u2010x in activated B cells, we tested their sensitivity to apoptosis induced by the Ca<jats:sup>2+<\/jats:sup> ATPase inhibitor thapsigargin: B cell blasts activated with anti\u2010CD40 and anti\u2010Ig were resistant to this agent. The available data therefore suggest that bcl\u2010x fulfils two roles in B cells: it promotes survival of immature B cells (which lack bcl\u20102) and secondly, it apparently plays an additional role in protecting activated mature B cells (perhaps those in germinal centers) from apoptotic stimuli.<\/jats:p>","DOI":"10.1002\/eji.1830260325","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T01:50:12Z","timestamp":1172800212000},"page":"676-682","source":"Crossref","is-referenced-by-count":29,"title":["Expression of bcl\u2010x during mouse B cell differentiation and following activation by various stimuli"],"prefix":"10.1002","volume":"26","author":[{"given":"Michael S. 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