{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T10:41:51Z","timestamp":1770720111812,"version":"3.49.0"},"reference-count":23,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2005,11,17]],"date-time":"2005-11-17T00:00:00Z","timestamp":1132185600000},"content-version":"vor","delay-in-days":3395,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1996,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human natural killer (NK) cells express inhibitory (p58) or activatory (p50) receptors for HLA\u2010C alleles. Here, we describe a novel member of the p58\/p50 family that is expressed by a subset of NK cells in about one third of donors. This molecule, termed p50.3, mediates NK cell triggering as revealed by the induction of intracellular free calcium mobilization, cytokine release and cytotoxicity. In addition, anti\u2010p50.3 monoclonal antibody (mAb) induced a selective, strong proliferation of p50.3<jats:sup>+<\/jats:sup> NK cells in peripheral blood lymphocytes. Although p50.3 molecules do not appear to display an obvious HLA class I specificity, they are usually coexpressed with known inhibitory receptors for HLA class I alleles. mAb\u2010mediated cross\u2010linking of these receptors leads to inhibition of the anti\u2010p50.3 mAb\u2010induced NK cell activation and proliferation. Surface p50.3 molecules are glycoproteins of \u223c 55\u201358 kDa which, upon deglycosylation, display a relative molecular mass of 36 kDa, similar to that of deglycosylated (activatory) p50 receptors. Analysis of the two\u2010dimensional peptide maps of the 50.3 molecules revealed a high homology with the other HLA\u2010C\u2010specific p58\/p50 receptors. The use of a set of oligodeoxynucleotide primers, previously shown to amplify the activatory (p50) forms of HLA\u2010C\u2010specific receptors, consistently amplified in p50.3<jats:sup>+<\/jats:sup> clones a cDNA sequence termed KKA3. This sequence belongs to the p58\/p50 multigene family, that encodes for a transmembrane protein specifically stained by anti\u2010p50.3 mAb in cell transfectants. Similar to p50 molecules, the KKA3\u2010encoded molecules are characterized by two extracellular immunoglobulin\u2010like domains, by the presence of a lysine in the transmembrane region and a short (39 amino acids) cytoplasmic tail which does not contain immune receptor tyrosine\u2010based activation motifs (ITAM)\u2010like sequences.<\/jats:p>","DOI":"10.1002\/eji.1830260823","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T02:39:17Z","timestamp":1172803157000},"page":"1816-1824","source":"Crossref","is-referenced-by-count":104,"title":["A novel surface molecule homologous to the p58\/p50 family of receptors is selectively expressed on a subset of human natural killer cells and induces both triggering of cell functions and proliferation"],"prefix":"10.1002","volume":"26","author":[{"given":"Cristina","family":"Bottino","sequence":"first","affiliation":[]},{"given":"Simona","family":"Sivori","sequence":"additional","affiliation":[]},{"given":"Massimo","family":"Vitale","sequence":"additional","affiliation":[]},{"given":"Claudia","family":"Cantoni","sequence":"additional","affiliation":[]},{"given":"Michela","family":"Falco","sequence":"additional","affiliation":[]},{"given":"Daniela","family":"Pende","sequence":"additional","affiliation":[]},{"given":"Luigia","family":"Morelli","sequence":"additional","affiliation":[]},{"given":"Raffaella","family":"Augugliaro","sequence":"additional","affiliation":[]},{"given":"Gianpietro","family":"Semenzato","sequence":"additional","affiliation":[]},{"given":"Roberto","family":"Biassoni","sequence":"additional","affiliation":[]},{"given":"Lorenzo","family":"Moretta","sequence":"additional","affiliation":[]},{"given":"Alessandro","family":"Moretta","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90466-2"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.11.040193.003145"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.14.1.619"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60513-1"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.176.4.963"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.178.2.597"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(90)90097-S"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.172.6.1589"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.171.3.695"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/1074-7613(95)90025-X"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7716543"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.182.3.875"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.2.645"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.23.8998"},{"key":"e_1_2_1_16_2","volume-title":"Molecular 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