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The efficiency of cleavage at the secretory poly(A) site is thought to play a crucial role in this alternative processing. We therefore examined RNA binding factors recognizing the secretory poly(A) site, in the absence of the splicing option, to look for transacting factors that may play a role in cleavage\/polyadenylation efficiency at this site. Purified primary B cells produce the secretory form of \u03bc mRNA when stimulated with lipopolysaccharide (LPS) and the membrane form of \u03bc mRNA when their antigen receptors are ligated by anti\u2010\u03bc antibodies. We compared RNA binding factors in nuclear extracts from cells produced by these different stimulatory conditions and show that induction of the secretory form of \u03bc mRNA by LPS correlates with the induction of a 28\u201332\u2010kDa secretory poly(A) site\u2010specific polypeptide which is also present in the plasmacytoma cell line J558L. Visualization of the 28\u201332\u2010kDa polypeptide in UV cross\u2010linking assays depends on a GU\u2010rich element downstream of the secretory poly (A) site. We show that this GU\u2010rich region enhances polyadenylation efficiency <jats:italic>in vivo<\/jats:italic> by transfection of luciferase reporter constructs into the plasmacytoma J558L. We also examined nuclear extracts from B cells doubly stimulated with LPS and anti\u2010\u03bc antibodies in which expression of the secretory form of \u03bc mRNA is selectively inhibited. This inhibition may be due to a down\u2010regulation of polyadenylation at the secretory poly(A) site or an up\u2010regulation of the competitive splicing process. This form of stimulation does not lead to the disappearance of the 28\u201332\u2010kDa polypeptide, but to an enhanced binding of a 50\u201355\u2010kDa factor which binds both the secretory and membrane poly(A) site. We report the first detection of changes in RNA binding factors taking place at the secretory poly(A) site which correlate with the expression of different forms of \u03bc mRNA produced by primary B cells under different stimulation conditions.<\/jats:p>","DOI":"10.1002\/eji.1830261247","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T01:29:25Z","timestamp":1172798965000},"page":"3144-3152","source":"Crossref","is-referenced-by-count":11,"title":["Inducible nuclear factors binding the IgM heavy chain pre\u2010mRNA secretory poly(A) site"],"prefix":"10.1002","volume":"26","author":[{"given":"Cathy","family":"Phillips","sequence":"first","affiliation":[]},{"given":"Anneliese","family":"Schimpl","sequence":"additional","affiliation":[]},{"given":"Walter","family":"Dietrich\u2010Goetz","sequence":"additional","affiliation":[]},{"given":"J. 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