{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T19:25:05Z","timestamp":1760297105182},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,2,25]],"date-time":"2004-02-25T00:00:00Z","timestamp":1077667200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2004,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The majority of tumor\u2010associated antigens are aberrantly expressed or overexpressed normal gene products. Therefore, mechanisms responsible for self tolerance dampen immune responses against these antigens. To evaluate the effect that tolerance has on the immune responses against tumor antigens, we characterized the CD8<jats:sup>+<\/jats:sup> T\u2004cell responses in neu mice. T\u2004cell responses against theA2.1\/neu p369\u2013377 and p773\u2013782 peptides were evaluated in neu mice that were crossed with A2.1\/Kb transgenic mice (A2\u00d7neu). Tetramer binding and cytotoxic activity demonstrate that, compared to CTL from A2.1\/Kb\u00d7FVB wild\u2010type mice (A2\u00d7FVB), CD8<jats:sup>+<\/jats:sup> T\u2004cells from A2\u00d7neu mice were of lower avidity for the peptides. Despite the fact that A2\u00d7neu mice are tolerant, multipleimmunizations with DC pulsed with the p369\u2013377 or p773\u2013782 peptides in the presence of IL\u20102 retarded tumor growth in A2\u00d7neu mice, and immunizations in combination with the anti\u2010OX40 mAb further enhanced the antitumor response. Taken together, these data indicate that low\u2010avidity T\u2004cells for neu antigens persisting in A2\u00d7neu mice have the capacity to develop antitumor responses as long as they are provided with efficient costimulation. These results underscore the potential role of low\u2010avidity T\u2004cells in antitumor immunity and may offer an important component for vaccination immunotherapies.<\/jats:p>","DOI":"10.1002\/eji.200324427","type":"journal-article","created":{"date-parts":[[2004,2,26]],"date-time":"2004-02-26T20:25:09Z","timestamp":1077827109000},"page":"752-761","source":"Crossref","is-referenced-by-count":57,"title":["The CD8<sup>+<\/sup> T\u2004cell repertoire against Her\u20102\/neu antigens in neu transgenic mice is of low avidity with antitumor activity"],"prefix":"10.1002","volume":"34","author":[{"given":"Joseph","family":"Lustgarten","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana\u2004Lucia","family":"Dominguez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camilo","family":"Cuadros","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,25]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90844-1"},{"key":"e_1_2_7_2_2","doi-asserted-by":"crossref","first-page":"1643","DOI":"10.1126\/science.1840703","article-title":"A\u2004gene encoding an antigen recognized by cytolytic T\u2004lymphocytes on a human melanoma.","volume":"254","author":"van der Bruggen P.","year":"1991","journal-title":"Science"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.2.1125"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80028-X"},{"key":"e_1_2_7_5_2","first-page":"281","article-title":"Augmentation of host immune responses to cancer: overcoming the barrier of tumor antigen\u2010specific T\u2004cell tolerance.","volume":"6","author":"Levitsky H. 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