{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T18:27:08Z","timestamp":1764872828852},"reference-count":34,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,3,11]],"date-time":"2005-03-11T00:00:00Z","timestamp":1110499200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In experimental autoimmune encephalomyelitis (EAE), intravenous transfer of activated CD4<jats:sup>+<\/jats:sup> myelin\u2010specific T cells is sufficient to induce disease. Transferred T cells access the CNS parenchyma by trafficking across the blood brain barrier (BBB) vascular endothelium into the perivascular space, and then across the glial limitans that is made up of astrocytes and microglia. Flow cytometry analysis of cells isolated from CNS tissue does not distinguish between T cell populations at the various stages of migration. In this study, we have used GK1.5 (anti\u2010CD4) treatment along with immunohistochemistry to distinguish between populations of T cells that are associated with the vasculature, T cells that have migrated into the perivascular space, and T cells in the parenchyma. We have also re\u2010evaluated antigen specificity requirements of T cells as they are recruited to the CNS parenchyma. Activated myelin\u2010specific T cells are restricted to the CNS vasculature for at least 24\u2004h post transfer. MHC class II expression on the recipient is required for cells to traffic across the CNS vascular endothelium. Further, Con A\u2010stimulated or non\u2010CNS\u2010specific (ovalbumin\u2010specific) T cells fail to migrate into the perivascular space, and only enter the CNS parenchyma when co\u2010transferred with myelin\u2010specific T cells. Our results indicate that Th1 populations cannot accumulate in the perivascular (subarachnoid, Virchow\u2010Robbins) space without a CNS antigen\u2010specific signal.<\/jats:p>","DOI":"10.1002\/eji.200425864","type":"journal-article","created":{"date-parts":[[2005,3,11]],"date-time":"2005-03-11T12:58:39Z","timestamp":1110545919000},"page":"1076-1085","source":"Crossref","is-referenced-by-count":53,"title":["Defining antigen\u2010dependent stages of T cell migration from the blood to the central nervous system parenchyma"],"prefix":"10.1002","volume":"35","author":[{"given":"Angela\u2004S.","family":"Archambault","sequence":"first","affiliation":[]},{"given":"Julia","family":"Sim","sequence":"additional","affiliation":[]},{"given":"Mary\u2004Ann\u2004T.","family":"Gimenez","sequence":"additional","affiliation":[]},{"given":"John\u2004H.","family":"Russell","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,3,22]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1111\/j.1750-3639.1996.tb00854.x","article-title":"Distinct patterns of multiple sclerosis pathology indicates heterogeneity on pathogenesis.","volume":"6","author":"Lucchinetti C. 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