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Three gel sections, each containing proteins previously detected as abundantly synthesized in tobacco mesophyll protoplasts and whose synthesis is reduced by auxin application, were excised from each gel and collected. These proteins were further separated on slab SDS gels and protein bands were excised after Coomassie Brilliant Blue R\u2010250 staining and used to inject three rabbits. After one booster, highly specific antibodies were detected in their sera by ELISA and immunoblotting. Using these sera we have confirmed that the corresponding proteins are identical in callus and mesophyll protoplast and demonstrated that they are abundantly accumulated in tobacco roots but are undetectable in aerial organs and seeds.<\/jats:p>","DOI":"10.1002\/elps.1150091105","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T08:57:37Z","timestamp":1117270657000},"page":"704-712","source":"Crossref","is-referenced-by-count":65,"title":["Preparation by two\u2010dimensional electrophoresis of proteins for antibody production: Antibodies against proteins whose synthesis is reduced by auxin in tobacco mesophyll protoplasts"],"prefix":"10.1002","volume":"9","author":[{"given":"Yves","family":"Meyer","sequence":"first","affiliation":[]},{"given":"Jean","family":"Grosset","sequence":"additional","affiliation":[]},{"given":"Yvette","family":"Chartier","sequence":"additional","affiliation":[]},{"given":"Jean\u2010Claude","family":"Cleyet\u2010Marel","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,4,13]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"1028","volume":"1984","author":"Meyer Y.","year":"1984","journal-title":"Plant Physiol."},{"key":"e_1_2_1_3_2","first-page":"1035","volume":"1984","author":"Meyer Y.","year":"1984","journal-title":"Plant Physiol."},{"doi-asserted-by":"publisher","key":"e_1_2_1_4_2","DOI":"10.1104\/pp.83.3.713"},{"doi-asserted-by":"publisher","key":"e_1_2_1_5_2","DOI":"10.1007\/BF00388868"},{"key":"e_1_2_1_6_2","first-page":"100","volume":"1985","author":"Murashige T.","year":"1985","journal-title":"Physiol. 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