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We will discuss the problems of classification and characterization of proteins and propose a systematic approach to the analysis of the total protein complex. Both a comprehensive as well as a pragmatic approach towards systematic analysis have been considered. A \u201ccomplex protein database\u201d is suggested and considered with regard to various uses. A systematic analysis of the mouse proteins has been started and some of the preliminary results are summarized here. In particular, genetic properties of the proteins were investigated and are presented in order to demonstrate the significance of a systematic analysis of proteins for research and practical application (<jats:italic>e. g.<\/jats:italic> mutagenicity testing). A concept is presented for sequencing the coding DNA of mouse and man, starting with a systematic analysis of mouse proteins and then using two recently developed methods \u2010 microsequencing of proteins from spots of 2\u2010DE protein patterns, and utilization of the relatively short <jats:italic>N<\/jats:italic>\u2010terminal sequences obtained \u2013 to produce the corresponding cDNA's of these proteins.<\/jats:p>","DOI":"10.1002\/elps.1150100208","type":"journal-article","created":{"date-parts":[[2005,5,27]],"date-time":"2005-05-27T20:20:21Z","timestamp":1117225221000},"page":"140-152","source":"Crossref","is-referenced-by-count":33,"title":["Systematic analysis of the total proteins of a mammalian organism: Principles, problems and implications for sequencing the human genome"],"prefix":"10.1002","volume":"10","author":[{"given":"J.","family":"Klose","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,4,13]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/BF00281458","volume":"26","author":"Klose J.","year":"1975","journal-title":"Humangenetik"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)41496-8"},{"key":"e_1_2_1_4_2","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1515\/9783111439846-004","volume-title":"Modern Methods in Protein Chemistry \u2013 Review Articles","author":"Klose J.","year":"1983"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-164720-9.50014-3"},{"key":"e_1_2_1_6_2","first-page":"1","volume":"1988","author":"Dunn M. 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