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The HIV is an unusually variable genome by virtue of a low replication fidelity. In this report we describe our effort to test the hypothesis that there is a correlation between virus variability and cytopathogenicity, and further, that there is an \u201cimpact\u201d of the virus infection on the expression of host cellular genes. To search for such a relationship, we infected H\u20109 cells (human CD4+ lymphoblastoid cell line) with each of 5 isolates of HIV of distinct origin and cytopathogenicity. To measure the influence of the virus infection on the expression of host cellular genes, shortly after infection, (3 h or 13 h), cells were radiolabeled and the radioactive polypeptides separated by two\u2010dimensional gel electrophoresis. Radiofluorographs were prepared and analyzed to determine relative rates of biosynthesis of cellular polypeptides. To organize the large amounts of data found, cluster analysis and principal component analysis were used to expose the data in formats that allowed a model construction. The rates of biosynthesis of many cellular polypeptides were altered upon viral infection in terms of both enhancements and impairment of biosynthesis. Some of the variation in polypeptide synthesis was isolate\u2010specific, while most alterations were of modest magnitude. There appears to be no \u201coverall effect\u201d associated with infection by a cytopathic variant of the virus. Polypeptides affected by the cytopathic variants were determined as targets for further investigation. The method used promotes the measurement of \u201censemble\u201d information that is characteristic of the process and it promotes the creation of models of virus action.<\/jats:p>","DOI":"10.1002\/elps.1150120715","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T09:25:45Z","timestamp":1117272345000},"page":"554-569","source":"Crossref","is-referenced-by-count":13,"title":["Global analysis of lymphocyte gene expression: Perturbation of H\u20109 cells by infection with distinct isolates of human immunodeficiency virus \u2013 an exposition by multivariate analysis of a host\u2010parasite interface"],"prefix":"10.1002","volume":"12","author":[{"given":"John R.","family":"Kettman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robin A.","family":"Robinson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lotte","family":"Kuhn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivan","family":"Lefkovits","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,4,13]]},"reference":[{"volume-title":"Numerical Taxonomy","year":"1973","author":"Sneath P. 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