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Furthermore, we have checked and supplemented existing data on p<jats:italic>K<\/jats:italic> values for the immobilized groups in the presence of 8M urea. This is necessary for pH gradients to be defined in a pH scale relevant to the focusing conditions such that spot positions can be related to amino acid compositions. The data will allow definition of pH scales for the temperature range 10\u201325\u00b0C and for a pH range covering the major part of the nonlinear pH gradient. With the latter, focusing positions are neither influenced by urea concentration nor by the choice or the concentration of detergent or carrier ampholyte. Temperature is the only parameter affecting focusing reproducibility and here any changes in focusing positions can be related to the amino acid compositions of peptides.<\/jats:p>","DOI":"10.1002\/elps.11501401209","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T09:32:37Z","timestamp":1117272757000},"page":"1357-1365","source":"Crossref","is-referenced-by-count":332,"title":["A nonlinear wide\u2010range immobilized pH gradient for two\u2010dimensional electrophoresis and its definition in a relevant pH scale"],"prefix":"10.1002","volume":"14","author":[{"given":"Bengt","family":"Bjellqvist","sequence":"first","affiliation":[]},{"given":"Christian","family":"Pasquali","sequence":"additional","affiliation":[]},{"given":"Florence","family":"Ravier","sequence":"additional","affiliation":[]},{"given":"Jean\u2010Charles","family":"Sanchez","sequence":"additional","affiliation":[]},{"given":"Denis","family":"Hochstrasser","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,4,13]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150090913"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150070205"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.11501301201"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150060113"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.11501401163"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)95297-5"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150040502"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-022X(82)90013-6"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150080207"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(88)90209-6"},{"key":"e_1_2_1_12_2","first-page":"35","volume":"1","author":"Hochstrasser D. 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