{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T21:28:48Z","timestamp":1762032528941,"version":"build-2065373602"},"reference-count":11,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,4,13]],"date-time":"2005-04-13T00:00:00Z","timestamp":1113350400000},"content-version":"vor","delay-in-days":4485,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[1993,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We describe some simple modifications to the micropreparative two\u2010dimensional (2\u2010D) electrophoresis procedure using immobilized pH gradients in the first dimension and sodium dodecyl sulfate\u2010electrophoresis in the second dimension. The geometry of the immobilized pH gradient strips has been changed to allow the use of large sample application cups that can accomodate greater sample volumes. The use of narrow range immobilized pH gradients with a large sample loading volume allows an efficient resolubilization of polypeptides after the first dimension. As a result, the vertical streaking caused by too high a protein concentration is eliminated in the second dimension. Protein identification by <jats:italic>N<\/jats:italic>\u2010terminal sequencing is facilitated by the large protein load (1\u201315 mg) which can be employed using this modification. Spots not normally detectable on conventional analytical 2\u2010D maps, even with sensitive silver staining, are observed. Results for plasma and liver proteins are shown.<\/jats:p>","DOI":"10.1002\/elps.11501401212","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T09:32:37Z","timestamp":1117272757000},"page":"1375-1378","source":"Crossref","is-referenced-by-count":162,"title":["Micropreparative two\u2010dimensional electrophoresis allowing the separation of samples containing milligram amounts of proteins"],"prefix":"10.1002","volume":"14","author":[{"given":"Bengt","family":"Bjellqvist","sequence":"first","affiliation":[]},{"given":"Jean\u2010Charles","family":"Sanchez","sequence":"additional","affiliation":[]},{"given":"Christian","family":"Pasquali","sequence":"additional","affiliation":[]},{"given":"Florence","family":"Ravier","sequence":"additional","affiliation":[]},{"given":"Nicole","family":"Paquet","sequence":"additional","affiliation":[]},{"given":"S\u00e9verine","family":"Frutiger","sequence":"additional","affiliation":[]},{"given":"Graham J.","family":"Hughes","sequence":"additional","affiliation":[]},{"given":"Denis","family":"Hochstrasser","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,4,13]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.11501401209"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.11501301150"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.11501301201"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.13.5709"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-022X(83)90040-4"},{"key":"e_1_2_1_7_2","unstructured":"Pharmacia LKB Application Note 324."},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150080207"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.11501301151"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150130190"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.11501401181"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.11501401163"}],"container-title":["ELECTROPHORESIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Felps.11501401212","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.11501401212","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T16:12:57Z","timestamp":1760544777000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elps.11501401212"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,1]]},"references-count":11,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1993,1]]}},"alternative-id":["10.1002\/elps.11501401212"],"URL":"https:\/\/doi.org\/10.1002\/elps.11501401212","archive":["Portico"],"relation":{},"ISSN":["0173-0835","1522-2683"],"issn-type":[{"type":"print","value":"0173-0835"},{"type":"electronic","value":"1522-2683"}],"subject":[],"published":{"date-parts":[[1993,1]]}}}