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The database, which contains some 1388 protein spots characterised in terms of <jats:italic>M<\/jats:italic><jats:sub>r<\/jats:sub> and p<jats:italic>I<\/jats:italic>, has been analysed further by Western immunoblotting and protein sequencing. From a total of 103 protein spots analysed, 49 have been identified by immunoblotting and 32 have been identified by protein sequencing. A further six proteins have tentatively been assigned by comparison with the human heart 2\u2010DE protein database of Jungblut <jats:italic>et al. (Electrophoresis<\/jats:italic>) 1994, <jats:italic>15<\/jats:italic>, 685\u2013607). This database is being used in studies of alterations in protein expression in the diseased and transplanted human heart.<\/jats:p>","DOI":"10.1002\/elps.11501501209","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T10:12:25Z","timestamp":1117275145000},"page":"1459-1465","source":"Crossref","is-referenced-by-count":55,"title":["The human myocardial two\u2010dimensional gel protein database: Update 1994"],"prefix":"10.1002","volume":"15","author":[{"given":"Joseph M.","family":"Corbett","sequence":"first","affiliation":[]},{"given":"Colin H.","family":"Wheeler","sequence":"additional","affiliation":[]},{"given":"Cathy S.","family":"Baker","sequence":"additional","affiliation":[]},{"given":"Magdi H.","family":"Yacoub","sequence":"additional","affiliation":[]},{"given":"Michael J.","family":"Dunn","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,4,13]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)41496-8"},{"key":"e_1_2_1_3_2","first-page":"1","volume":"1","author":"Dunn M. 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