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Amino acid composition was used to putatively identify, or provide unique parameters, for 50 gene products separated by two\u2010dimensional gel electrophoresis. A further 19 proteins were subjected to peptide\u2010mass fingerprinting using matrix\u2010assisted laser desorption ionisation\u2010time of flight (MALDI\u2010TOF) mass spectrometry and 4 were subjected to <jats:italic>N<\/jats:italic>\u2010terminal Edman degradation. The majority of <jats:italic>M. genitalium<\/jats:italic> proteins remain uncharacterised. However, the combined approach of amino acid analysis and peptide\u2010mass fingerprinting allowed gene products to be linked to homologous genes in a variety of organisms. This has allowed proteins to be identified prior to detection of their respective genes <jats:italic>via<\/jats:italic> the <jats:italic>M. genitalium<\/jats:italic> sequencing initiative. The principle of \u2018hierarchical\u2019 analysis for the mass screening of proteins and the analysis of microbial genomes <jats:italic>via<\/jats:italic> their protein complement or \u2018proteome\u2019 is detailed. Here, characterisation of gene products depends upon the quickest and most economical technologies being employed initially, so as to determine if a large number of proteins are already present in both homologous and heterologous species databases. Initial screening, which lends itself to automation and robotics, can then be followed by more time and cost intensive procedures, when necessary.<\/jats:p>","DOI":"10.1002\/elps.11501601185","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T10:29:35Z","timestamp":1117276175000},"page":"1090-1094","source":"Crossref","is-referenced-by-count":752,"title":["Progress with gene\u2010product mapping of the Mollicutes: <i>Mycoplasma genitalium<\/i>"],"prefix":"10.1002","volume":"16","author":[{"given":"Valerie C.","family":"Wasinger","sequence":"first","affiliation":[]},{"given":"Stuart J.","family":"Cordwell","sequence":"additional","affiliation":[]},{"given":"Anne","family":"Cerpa\u2010Poljak","sequence":"additional","affiliation":[]},{"given":"Jun X.","family":"Yan","sequence":"additional","affiliation":[]},{"given":"Andrew A.","family":"Gooley","sequence":"additional","affiliation":[]},{"given":"Marc R.","family":"Wilkins","sequence":"additional","affiliation":[]},{"given":"Mark W.","family":"Duncan","sequence":"additional","affiliation":[]},{"given":"Ray","family":"Harris","sequence":"additional","affiliation":[]},{"given":"Keith L.","family":"Williams","sequence":"additional","affiliation":[]},{"given":"Ian","family":"Humphery\u2010Smith","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,4,14]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.24.7918-7930.1993"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(94)90853-2"},{"key":"e_1_2_1_4_2","first-page":"338","volume":"3","author":"Hilbert H.","year":"1994","journal-title":"IOM Letters"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150160171"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.11.5138"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1994.1474"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150150197"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.11.5011"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0960-9822(93)90195-T"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1993.2009"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/bms.1200220605"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00091a044"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/bms.1200230503"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1993.1514"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150160151"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00096a002"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.11501501183"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.11501401209"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.11501401212"},{"key":"e_1_2_1_21_2","doi-asserted-by":"crossref","first-page":"6455","DOI":"10.1128\/jb.171.12.6455-6467.1989","volume":"171","author":"Tully J. 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