{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T19:46:23Z","timestamp":1771011983188,"version":"3.50.1"},"reference-count":47,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,7,14]],"date-time":"2006-07-14T00:00:00Z","timestamp":1152835200000},"content-version":"vor","delay-in-days":4942,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Environ and Mol Mutagen"],"published-print":{"date-parts":[[1993,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The occurrence of deletions, coding sequence alterations, and intronic changes leading to aberrant splicing has been characterized among 33 spontaneous HPRT<jats:sup>\u2212<\/jats:sup> mutants in TK6 human lymphoblasts. Deletions detectable by multiplex PCR amplification accounted for 45% (15\/33) of the mutant collection. Base substitutions represented 30% (10\/33) of the total, and were predominated by changes at G:C base pairs. The remaining mutants were distributed among frameshifts (9%, 3\/33), small deletions (6%, 2\/33), and compound alterations (9%, 3\/33). Five mutants (15%) demonstrated aberrant splicing of the <jats:italic>hprt<\/jats:italic> transcript. A cluster of 4 deletion\/insertion events was identified in <jats:italic>hprt<\/jats:italic> exon 6. A nearly perfect 13 bp duplication differed from the original sequence only by an A:T to G:C transition, which was observed as a unique alteration in another HPRT<jats:sup>\u2212<\/jats:sup> mutant. A model involving correction of a mismatch in a secondary structure formed by the duplicated sequence may account for these results. \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/em.2850220305","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T19:04:56Z","timestamp":1172084696000},"page":"138-146","source":"Crossref","is-referenced-by-count":17,"title":["Spectrum of spontaneous HPRT mutations in TK6 human lymphoblasts"],"prefix":"10.1002","volume":"22","author":[{"given":"Cynthia R.","family":"Giver","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen L.","family":"Nelson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew J.","family":"Grosovsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,14]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(82)90148-9"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2136249"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(91)90157-J"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/mc.2940030405"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/em.2850200202"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/287560a0"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(80)90089-6"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0888-7543(90)90493-E"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00329839"},{"key":"e_1_2_1_11_1","first-page":"6001","article-title":"V(D)J recombinase\u2010like activity mediates hprt gene deletion in human fetal T\u2010lymphocytes","volume":"51","author":"Fuscoe JC","year":"1991","journal-title":"Cancer Res"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-7992(92)90057-O"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(88)90121-2"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.6.1919"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0888-7543(90)90545-6"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.2.512"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1905840"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/8.1.43"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00259606"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.2.477"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-349-20235-5_8"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(82)90308-6"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1110(90)90022-4"},{"key":"e_1_2_1_24_1","first-page":"5489","article-title":"Endogenous carcinogenesis: Molecular oncology into the twenty\u2010first century\u2010presidential address","volume":"49","author":"Loeb LA","year":"1989","journal-title":"Cancer Res"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1110(90)90021-3"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1110(90)90020-C"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01233170"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0888-7543(92)90153-J"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-7992(83)90165-3"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.13.6177"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/em.2850130403"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.6.2.393"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/5.5.505"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/em.2860080511"},{"key":"e_1_2_1_35_1","first-page":"241","article-title":"ras and human tumors","volume":"3","author":"Rodenhuis S","year":"1992","journal-title":"Semin Cancer Biol"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-7992(90)90084-W"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/15.17.7155"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1110(92)90002-Q"},{"key":"e_1_2_1_39_1","doi-asserted-by":"crossref","unstructured":"SmithLE GrosovskyAJ(1993):Evidence for high frequency allele loss at the aprt locus in TK6 human lymphoblasts. 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