{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T03:07:16Z","timestamp":1779160036837,"version":"3.51.4"},"reference-count":68,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,7,14]],"date-time":"2006-07-14T00:00:00Z","timestamp":1152835200000},"content-version":"vor","delay-in-days":4577,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Environ and Mol Mutagen"],"published-print":{"date-parts":[[1994,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cells from patients with ataxia\u2010telangiectasia (AT) are more sensitive than cells from normal individuals to a number of compounds which induce DNA damage via oxygen\u2010derived free radical attack. We tested the hypothesis that AT cells would show a sensitivity to reactive oxygen species (ROS) generated by activated inflammatory cells. AT cells were exposed to neutro\u2010phils activated with 12\u2010O\u2010tetradecanoyl\u2010phor\u2010bol\u201013\u2010acetate (TPA) or to xanthine\/xanthine oxidase (X\/XO), an enzyme system which generates superoxide and hydrogen peroxide. Induced micronuclei (MN) frequencies (corrected for spontaneous MN frequencies) were significantly higher in AT cell cultures than in cultures from normal individuals (comparison of MN frequencies of AT vs. normal cultures: for treatment with activated neutrophils, <jats:italic>P<\/jats:italic> = 0.003; for X\/XO, <jats:italic>P<\/jats:italic> = 0.05). The comet assay was used to determine whether the elevated chromosomal damage in the treated AT cells was due to a difference in strand breakage or its rejoining. X\/XO treatment was used in studies of single\u2010stranded (SS) DNA breakage, and X\u2010ray treatment for double\u2010stranded (DS) DNA damage. AT and normal cells showed no significant differences in the initial levels of SS (<jats:italic>P<\/jats:italic> = 0.29) or DS (<jats:italic>P<\/jats:italic> = 0.91) DNA damage. Likewise, they exhibited similar rejoining kinetics (rejoining half\u2010time for SS = 10 min, for DS = 30 min). These data support the involvement of the AT loci in determining a cell's ability to deal with oxidative stress, although the mechanism underlying this effect has yet to be resolved. The data also suggest that AT patients are at elevated risk of sustaining DNA damage in tissues undergoing inflammatory reactions. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/em.2850240205","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T19:17:50Z","timestamp":1172085470000},"page":"103-111","source":"Crossref","is-referenced-by-count":47,"title":["Response of fibroblast cultures from ataxia\u2010telangiectasia patients to reactive oxygen species generated during inflammatory reactions"],"prefix":"10.1002","volume":"24","author":[{"given":"A. J.","family":"Ward","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. L.","family":"Olive","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. H.","family":"Burr","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. P.","family":"Rosin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,14]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0921-8777(89)90023-2"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6276978"},{"key":"e_1_2_1_4_1","first-page":"539","volume-title":"Radioprotectors and Anticarcinogens","author":"Birnboim HC","year":"1983"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/09553009114552601"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0921-8777(89)90020-7"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsscb.380170408"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/09553008714550711"},{"key":"e_1_2_1_9_1","first-page":"5374","article-title":"Correspondence re: Michael J. Meredith and Marion L. Dodson. Impaired glutathione biosynthesis in cultured Ataxia\u2010Telangiectasia cells","volume":"48","author":"Dean SW","year":"1988","journal-title":"Cancer Res"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.1984.Supplement_6.12"},{"key":"e_1_2_1_11_1","first-page":"1269","article-title":"Modification of DMA bases in chromatin of intact target human cells by activated human polymorphonuclear leukocytes","volume":"53","author":"Dizdaroglu M","year":"1993","journal-title":"Cancer Res"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/6.9.1279"},{"key":"e_1_2_1_13_1","first-page":"67","article-title":"The measurement of opsonic and phagocytic function by Luminol\u2010dependent chemilu\u2010minescence","volume":"41","author":"Easmon CSF","year":"1980","journal-title":"Immunology"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1161(85)90015-9"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2787(80)90153-7"},{"key":"e_1_2_1_16_1","first-page":"1703","article-title":"Repair of ionizing radiation DNA base damage in Ataxia\u2010telangiectasia cells","volume":"46","author":"Fornace AJ","year":"1986","journal-title":"Cancer Res"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/8.3.455"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0163-7258(92)90047-4"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1218(93)90101-I"},{"key":"e_1_2_1_20_1","first-page":"127","article-title":"Candidates for the molecular defect in ataxia telangiectasia","volume":"61","author":"Gatti RA","year":"1993","journal-title":"Adv Neurol"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0079-6107(87)90012-5"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0921-8734(92)90043-O"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4684-5496-3_13"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.2307\/3575473"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0163-7258(92)90057-7"},{"key":"e_1_2_1_26_1","first-page":"19","article-title":"Cancer complicating chronic ulcerative and scarifying mucocutaneous disorders","volume":"2","author":"Kaplan RP","year":"1987","journal-title":"Adv Dermatol"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90593-2"},{"key":"e_1_2_1_28_1","first-page":"173","volume-title":"Cellular and Molecular Targets for Chemoprevention","author":"Kensler TW","year":"1992"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0748-5514(85)90159-X"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0921-8777(92)90005-N"},{"key":"e_1_2_1_31_1","first-page":"1270","article-title":"Induction of 5, 6\u2010ring saturated thymine bases in NIH\u20103T3 cells by phorbol ester\u2010stimulated macrophages: role of reactive oxygen intermediates","volume":"45","author":"Lewis JG","year":"1985","journal-title":"Cancer Res"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/7.5.813"},{"key":"e_1_2_1_33_1","first-page":"4576","article-title":"Impaired glutathione biosynthesis in cultures of human ataxia\u2010telangiectasia cells","volume":"47","author":"Meredith MJ","year":"1987","journal-title":"Cancer Res"},{"key":"e_1_2_1_34_1","first-page":"67","article-title":"DNA replication and repair in ataxia telangiectasia cells exposed to bleomycin","volume":"112","author":"Morris C","year":"1983","journal-title":"Mutat Res"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1080\/09553008914551861"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.14.6338"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.4.990"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.2307\/3577587"},{"key":"e_1_2_1_39_1","first-page":"4671","article-title":"DNA double\u2010strand breaks measured in individual cells subjected to gel electrophoresis","volume":"51","author":"Olive PL","year":"1991","journal-title":"Cancer Res"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.2307\/3578485"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.2307\/3578443"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00219338"},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/0921-8777(93)90053-J"},{"key":"e_1_2_1_44_1","first-page":"7415","article-title":"Increased cell division as a cause of human cancer","volume":"50","author":"Preston\u2010Martin S","year":"1990","journal-title":"Cancer Res"},{"key":"e_1_2_1_45_1","doi-asserted-by":"publisher","DOI":"10.2307\/3577847"},{"key":"e_1_2_1_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(92)90071-9"},{"key":"e_1_2_1_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(94)90248-8"},{"key":"e_1_2_1_48_1","unstructured":"RosinMP AnwarWA WardAJ(1994):Inflammation chromosomal instability and cancer: the schistosomiasis model. Cancer Res (in press)."},{"key":"e_1_2_1_49_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/82.12.1050"},{"key":"e_1_2_1_50_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/9.12.2297"},{"key":"e_1_2_1_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(79)90141-6"},{"key":"e_1_2_1_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/4.10.1317"},{"key":"e_1_2_1_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00210663"},{"key":"e_1_2_1_54_1","doi-asserted-by":"publisher","DOI":"10.1080\/09553008514550941"},{"key":"e_1_2_1_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/258427a0"},{"key":"e_1_2_1_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1110(89)90024-9"},{"key":"e_1_2_1_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1110(88)90022-X"},{"key":"e_1_2_1_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1110(87)90014-5"},{"key":"e_1_2_1_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/0921-8777(93)90012-6"},{"key":"e_1_2_1_60_1","first-page":"4443","article-title":"In vivo formation of oxidzxed DNA bases in tumour promoter\u2010treated mouse skin","volume":"51","author":"Wei H","year":"1991","journal-title":"Cancer Res"},{"key":"e_1_2_1_61_1","first-page":"1298","article-title":"Suppression of tumor promoter\u2010induced oxidative events and DNA damage in vivo by sarcophytol A: a possible mechanism of anti\u2010promotion","volume":"52","author":"Wei H","year":"1992","journal-title":"Cancer Res"},{"key":"e_1_2_1_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1218(89)90002-5"},{"key":"e_1_2_1_63_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V76.4.655.655"},{"key":"e_1_2_1_64_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6259738"},{"key":"e_1_2_1_65_1","doi-asserted-by":"crossref","first-page":"2770","DOI":"10.4049\/jimmunol.128.6.2770","article-title":"Effect of oxygen radical scavengers and anti\u2010oxidants on phagocyte\u2010induced mutagenesis","volume":"128","author":"Weitzman SA","year":"1982","journal-title":"J Immunol"},{"key":"e_1_2_1_66_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3975611"},{"key":"e_1_2_1_67_1","first-page":"2396","article-title":"Macrophage\u2010mediated induction of drug resistant variants in a mouse mammary tumor cell line","volume":"46","author":"Yamashina K","year":"1986","journal-title":"Cancer Res"},{"key":"e_1_2_1_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3835(90)90089-G"},{"key":"e_1_2_1_69_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.7.2085"}],"container-title":["Environmental and Molecular Mutagenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fem.2850240205","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/em.2850240205","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T19:33:57Z","timestamp":1698176037000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/em.2850240205"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,1]]},"references-count":68,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1994,1]]}},"alternative-id":["10.1002\/em.2850240205"],"URL":"https:\/\/doi.org\/10.1002\/em.2850240205","archive":["Portico"],"relation":{},"ISSN":["0893-6692","1098-2280"],"issn-type":[{"value":"0893-6692","type":"print"},{"value":"1098-2280","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,1]]}}}