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Our findings allow us to suggest a series of modifications of existing culturing and chromosome preparation techniques for breast cancer cytogenetic analysis. The improvements include: (1) combined mechanical and enzymatic disaggregation of the tumor samples, (2) initiation of short\u2010term cultures in plastic flasks that have a Primaria\u2010modified tissue culture surface or have been coated with Vitrogen 100, (3) use of serum\u2010free growth medium, CDM\u20105, but with temporary (24 hours) enrichment with 20% FBS if rapid cell attachment is not achieved, (4) partial and sequential harvesting of the cultures, and (5) use of minimal volumes of hypotonic and fixative solutions during harvesting.<\/jats:p>","DOI":"10.1002\/gcc.2870050103","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T23:10:43Z","timestamp":1172099443000},"page":"14-20","source":"Crossref","is-referenced-by-count":80,"title":["Improved technique for short\u2010term culture and cytogenetic analysis of human breast 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