{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T22:32:50Z","timestamp":1762641170232},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,14]],"date-time":"2006-07-14T00:00:00Z","timestamp":1152835200000},"content-version":"vor","delay-in-days":4608,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[1993,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have recently cloned the human cDNA for a gene, denoted <jats:italic>D4<\/jats:italic>, that encodes a protein 67% identical to the bovine rhoGDI protein, a GDP dissociation inhibitor (GDI) for the ras\u2010related rho\u2010subtype proteins. We now present data on the cloning and structural analysis of the murine <jats:italic>D4<\/jats:italic> cDNA and confirm its preferential expression in hematopoietic tissues. The predicted murine and human D4 proteins are almost 90% identical, indicating that <jats:italic>D4<\/jats:italic> and <jats:italic>rhoGDI<\/jats:italic> are different genes and that they are probably members of a related family of genes. Functional studies with the human D4 protein demonstrate that D4 has GDI activity against the CDC42Hs and rac I proteins, but binds to these proteins with a significantly weaker affinity than does the rho\u2010subtype GDI. These data suggest that D4, which will in subsequent communications be denoted as GDI.D4, might be a GDI for other known or as yet unidentified ras\u2010like GTP\u2010binding proteins. Alternatively, D4 could have other biochemical functions. During murine embryogenesis, <jats:italic>D4<\/jats:italic> transcripts are detected in yolk\u2010sac cells, where the earliest hematopoietic precursors are found. When these precursors undergo proliferation and differentiation in vitro, a dramatic increase in <jats:italic>D4<\/jats:italic> expression is seen. <jats:italic>D4<\/jats:italic> probably has a significant function during the growth and development of hematopoietic precursors. \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gcc.2870080408","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T23:51:58Z","timestamp":1172101918000},"page":"253-261","source":"Crossref","is-referenced-by-count":36,"title":["Identification of a novel protein with GDP dissociation inhibitor activity for the ras\u2010like proteins CDC42Hs and Rac 1"],"prefix":"10.1002","volume":"8","author":[{"given":"Chaker N.","family":"Adra","sequence":"first","affiliation":[]},{"given":"Jon","family":"Ko","sequence":"additional","affiliation":[]},{"given":"David","family":"Leonard","sequence":"additional","affiliation":[]},{"given":"Lori 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characterization of a GDP\u2010dissociation inhibitor (GDI) for the CDC42Hs protein","volume":"267","author":"Leonard D","year":"1992","journal-title":"J Biol Chem"},{"key":"e_1_2_1_19_1","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1242\/dev.113.4.1315","article-title":"In vitro development of murine T cells from pre\u2010thymic and pre\u2010liver embryonic yolk sac hematopoietic stem cells","volume":"113","author":"Liu C\u2010P","year":"1991","journal-title":"Development"},{"key":"e_1_2_1_20_1","volume-title":"Haemopoietic Cells","author":"Metcalf D","year":"1971"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/362462a0"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.8.2444"},{"key":"e_1_2_1_23_1","doi-asserted-by":"crossref","first-page":"2016","DOI":"10.1182\/blood.V77.9.2016.2016","article-title":"New recurring chromosomal translocations in childhood acute lymphoblastic leukemia","volume":"77","author":"Raimondi 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GDP from and the subsequent binding of GTP to rhoBp20, a ras p21\u2010like GTP\u2010binding protein","volume":"265","author":"Ueda T","year":"1990","journal-title":"J Biol Chem"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.11.3851"}],"container-title":["Genes, Chromosomes and 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