{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T01:08:38Z","timestamp":1760404118508},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2003,10,8]],"date-time":"2003-10-08T00:00:00Z","timestamp":1065571200000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2003,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The gene transduction technique is a useful method to study gene functions that underlie vertebrate embryogenesis. In this study, a new gene transduction technique is reported using microbubble\u2010enhanced sonoporation (hereafter referred to as sonoporation) to achieve ectopic and transient gene expression for several embryonic organs including embryonic chick limb bud mesenchymes. The technique proposed in this study has the advantages of 1) relatively simple gene transduction procedures, and 2) efficient exogenous gene transduction and expression with lower damages to embryos. Green fluorescent protein (GFP) or LacZ was misexpressed in limb bud mesenchymes by sonoporation, with the introduced expression transiently detected in the injected sites. Most of the transduced chick embryos survived without showing significant embryonic abnormalities or cell death after sonoporation. To demonstrate its efficacy for assessing the effect of transient gene transduction, the <jats:italic>Shh<\/jats:italic> (<jats:italic>sonic hedgehog<\/jats:italic>) was transduced into the developing chick limb bud. The transduced limb bud displayed limb malformations including partial digit duplication. Advantages and possible future applications in relation to this method are discussed. genesis 37:91\u2013101, 2003. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gene.10232","type":"journal-article","created":{"date-parts":[[2003,10,29]],"date-time":"2003-10-29T10:00:32Z","timestamp":1067421632000},"page":"91-101","source":"Crossref","is-referenced-by-count":48,"title":["Microbubble\u2010enhanced sonoporation: Efficient gene transduction technique for chick embryos"],"prefix":"10.1002","volume":"37","author":[{"given":"Sho","family":"Ohta","sequence":"first","affiliation":[]},{"given":"Kentaro","family":"Suzuki","sequence":"additional","affiliation":[]},{"given":"Katsuro","family":"Tachibana","sequence":"additional","affiliation":[]},{"given":"Gen","family":"Yamada","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2003,10,8]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3301302"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"5127","DOI":"10.1242\/dev.126.22.5127","article-title":"Engrailed defines the position of dorsal di\u2010mesencephalic boundary by repressing diencephalic fate","volume":"126","author":"Araki I","year":"1999","journal-title":"Development"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/hc3001.092038"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-5629(97)00025-2"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"1337","DOI":"10.1242\/dev.127.7.1337","article-title":"A model for anteroposterior patterning of the vertebrate limb based on sequential long\u2010 and short\u2010range Shh signalling and Bmp signalling","volume":"127","author":"Drossopoulou G","year":"2000","journal-title":"Development"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1006\/mthe.2002.0577"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-5629(02)00700-7"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-169x.2000.00504.x"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1050880104"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/350585a0"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(02)00291-5"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(99)00262-2"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.280.5367.1274"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.1996.7.11-1339"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-5629(00)00200-3"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3301339"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1996.0300"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(89)90209-6"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-679X(08)60629-9"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1155"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1540-8175.2001.00339.x"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.10104"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-3806(99)00073-5"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.126.5.883"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.128.22.4463"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90626-2"},{"key":"e_1_2_1_28_1","doi-asserted-by":"crossref","first-page":"3925","DOI":"10.1242\/dev.125.20.3925","article-title":"Lhx2, a vertebrate homologue of apterous, regulates vertebrate limb outgrowth","volume":"125","author":"Rodriguez Esteban C","year":"1998","journal-title":"Development"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3300711"},{"key":"e_1_2_1_30_1","first-page":"78","volume-title":"Ectodermal\u2010mesenchymal interactions","author":"Saunders JW","year":"1968"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.101.22.2554"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.92.5.1148"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1540-8175.2001.00323.x"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-0436.2002.700405.x"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/19762"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/416527a"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1161\/hc1002.105228"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3301678"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/289295a0"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/gene.1015"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1999.4761"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(02)00151-X"},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004424-200001000-00010"},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1021\/js9504752"},{"key":"e_1_2_1_45_1","first-page":"1165","article-title":"An efficient liposome\u2010mediated gene transfer into the branchial arch, neural tube and the heart of chick embryos; a strategy to elucidate the organogenesis","volume":"43","author":"Yamada G","year":"1997","journal-title":"Cell Mol Biol (Noisy le grand)"},{"key":"e_1_2_1_46_1","doi-asserted-by":"crossref","first-page":"4393","DOI":"10.1242\/dev.124.21.4393","article-title":"Relationship between dose, distance and time in Sonic Hedgehog\u2010mediated regulation of anteroposterior polarity in the chick limb","volume":"124","author":"Yang Y","year":"1997","journal-title":"Development"},{"key":"e_1_2_1_47_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-169x.2000.00502.x"},{"key":"e_1_2_1_48_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-169x.2000.00500.x"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fgene.10232","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/gene.10232","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T18:32:35Z","timestamp":1700159555000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/gene.10232"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,10]]},"references-count":47,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2003,10]]}},"alternative-id":["10.1002\/gene.10232"],"URL":"https:\/\/doi.org\/10.1002\/gene.10232","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"value":"1526-954X","type":"print"},{"value":"1526-968X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,10]]}}}