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HeLa cells expressing Panx1 have cell surface labeling, but not gap junction plaques, and do not transfer small fluorescent dyes or neurobiotin in a scrape\u2010loading assay. <jats:italic>Neuro<\/jats:italic>2a cells expressing Panx1 are not electrophysiologically coupled. Intracellular Panx1\u2010immunoreactivity, but not gap junction plaques, is seen in cultured oligodendrocytes, astrocytes, and hippocampal neurons. Thus, at least in these mammalian cells lines, Panx1 does not form morphological or functional gap junctions, and it remains to be demonstrated that Panx1 forms gap junction\u2010forming protein in the CNS. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/glia.20435","type":"journal-article","created":{"date-parts":[[2006,9,28]],"date-time":"2006-09-28T16:30:27Z","timestamp":1159461027000},"page":"46-56","source":"Crossref","is-referenced-by-count":158,"title":["Pannexin1 is expressed by neurons and glia but does not form functional gap junctions"],"prefix":"10.1002","volume":"55","author":[{"given":"Yan","family":"Huang","sequence":"first","affiliation":[]},{"given":"Judith B.","family":"Grinspan","sequence":"additional","affiliation":[]},{"given":"Charles K.","family":"Abrams","sequence":"additional","affiliation":[]},{"given":"Steven 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