{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T05:49:12Z","timestamp":1761630552388,"version":"3.30.0"},"reference-count":28,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":12176,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1982,5]]},"abstract":"\n \n The formation and clearance of circulating IgA immune complexes from blood to bile was investigated in this study. The i.v. injection of either MOPC\u2013315, an IgA M\u2013component with anti\u2013dinitrophenyl (DNP) specificity, or TEPC\u201315, an IgA M\u2013component of a different specificity, was followed by i.v. injection of 125<\/jats:sup>I\u2013DNP10<\/jats:sub>\u2013bovine serum albumin (BSA) as the antigen. The formation and clearance of IgA immune complexes in the circulation of MOPC\u2013315\u2013treated, but not TEPC\u201315\u2013treated animals was de\u00f8monstrated by immunoprecipitation with polyacrylamide beads coated with rabbit anti\u2013mouse IgA. IgA\u2013125<\/jats:sup>I\u2013DNP10<\/jats:sub>\u2013BSA complexes were identified in the bile from MOPC\u2013315\u2013treated, but not TEPC\u201315\u2013treated animals utilizing this same immunoprecipitation technique. These observations suggest that the liver or bile ducts transport IgA immune complexes from blood into bile. The clearance of 125<\/jats:sup>I\u2013DNP10<\/jats:sub>\u2013BSA from the circulation was documented by coprecipitation with rabbit anti\u2013BSA and BSA. The clearance of this circulating antigen was slower in the MOPC\u2013315\u2013treated than in the TEPC\u201315\u2013treated animals suggesting that under the conditions of the present experiment, circulating antigen is cleared more slowly after IgA immune complex formation.<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/hep.1840020307","type":"journal-article","created":{"date-parts":[[2010,7,5]],"date-time":"2010-07-05T12:53:54Z","timestamp":1278334434000},"page":"328S-333S","update-policy":"http:\/\/dx.doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":30,"title":["Hepatobiliary Clearance of Iga Immune Complexes Formed in the Circulation"],"prefix":"10.1097","volume":"2","author":[{"given":"Paul R.","family":"Harmatz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronald E.","family":"Kleinman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruce W.","family":"Bunnell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kurt J.","family":"Bloch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allan W.","family":"Walker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"10.1002\/hep.1840020307-BIB1|cit1","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1016\/S0016-5085(74)80174-5","article-title":"Protein uptake by the intestine: evidence for absorption of intact macromolecules","volume":"66","author":"Warshaw","year":"1974","journal-title":"Gastroenterology"},{"key":"10.1002\/hep.1840020307-BIB2|cit2","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/0022-1759(80)90319-1","article-title":"Solid phase radioimmunoassay for detection of circulating food protein antigens in human serum","volume":"37","author":"Paganelli","year":"1980","journal-title":"J Immunol Methods"},{"key":"10.1002\/hep.1840020307-BIB3|cit3","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1016\/S0016-5085(19)32855-0","article-title":"Uptake and transport of macromolecules by the intestine: possible role in clinical disorders","volume":"67","author":"Walker","year":"1974","journal-title":"Gastroenterology"},{"key":"10.1002\/hep.1840020307-BIB4|cit4","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1016\/S0016-5085(79)80075-X","article-title":"Intestinal uptake of macromolecules. 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