{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T02:19:49Z","timestamp":1769221189500,"version":"3.49.0"},"reference-count":23,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":11566,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1984,1]]},"abstract":"<jats:sec>\n            <jats:title\/>\n            <jats:p>Previous studies demonstrated that peripheral blood lymphocytes are cytotoxic to autologous hepatocytes in patients with chronic hepatitis B virus infection. We examined whether cytotoxicity is specifically directed against hepatocytes expressing HBsAg or HBcAg. Viral antigens were detected by immunofluorescence in isolated hepatocytes before and after exposure to T and non-T lymphocytes from 28 patients with chronic HBV infection in an autologous cytotoxicity assay.<\/jats:p>\n            <jats:p>There was significant reduction in the percentage of HBcAg-positive hepatocytes after exposure to T lymphocytes and, to a lesser extent, after exposure to non-T cells; hepatocytes expressing HBsAg were not affected. Other studies showed that many hepatocytes containing HBcAg have IgG of anti-HBc specificity bound to their nuclei. In the present study, there was significant association between the presence of HBcAg and nuclear IgG in isolated hepatocytes. The ratio between the percentages of HBcAg-positive and IgG-positive hepatocytes (reflecting the proportion of hepatocytes containing free-core antigen) correlated significantly with reduction in HBcAg-positive liver cells after exposure to T cells, but not to non-T cells.<\/jats:p>\n            <jats:p>These results suggest that hepatocytes in which viral replication is occurring and which express core determinants are susceptible to cell-mediated cytotoxicity and that T-cell cytolysis may be modulated by anti-HBc which is bound to hepatocytes.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1002\/hep.1840040111","type":"journal-article","created":{"date-parts":[[2008,8,22]],"date-time":"2008-08-22T13:52:03Z","timestamp":1219413123000},"page":"63-68","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":115,"title":["Relationship Between Expression of Hepatitis B Virus Antigens in Isolated Hepatocytes and Autologous Lymphocyte Cytotoxicity in Patients with Chronic Hepatitis B Virus Infection"],"prefix":"10.1097","volume":"4","author":[{"given":"Nikolai V.","family":"Naumov","sequence":"first","affiliation":[]},{"given":"Mario","family":"Mondelli","sequence":"additional","affiliation":[]},{"given":"Graeme J. M.","family":"Alexander","sequence":"additional","affiliation":[]},{"given":"Richard S.","family":"Tedder","sequence":"additional","affiliation":[]},{"given":"Adrian L. W. F.","family":"Eddleston","sequence":"additional","affiliation":[]},{"given":"Roger","family":"Williams","sequence":"additional","affiliation":[]}],"member":"276","reference":[{"key":"10.1002\/hep.1840040111-BIB1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1097\/00000441-197509000-00001","article-title":"Immunological aspects of hepatitis B virus infection","volume":"270","author":"Edgington","year":"1975","journal-title":"Am J Med Sci"},{"key":"10.1002\/hep.1840040111-BIB2","first-page":"1009","volume-title":"Clinical immunology","author":"Eddleston","year":"1980"},{"key":"10.1002\/hep.1840040111-BIB3","first-page":"161","volume-title":"Virus and the liver","author":"Thomas","year":"1980"},{"key":"10.1002\/hep.1840040111-BIB4","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1111\/j.1600-0676.1982.tb00211.x","article-title":"Heterogeneity of antigenic expression at the liver cell surface in hepatitis B virus infection","volume":"2","author":"Realdi","year":"1982","journal-title":"Liver"},{"key":"10.1002\/hep.1840040111-BIB5","first-page":"85","volume-title":"Virus and the liver","author":"Nazarewicz-de Mezer","year":"1980"},{"key":"10.1002\/hep.1840040111-BIB6","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1016\/S0016-5085(19)31906-7","article-title":"Evidence for phasic sequences in nuclear HBcAg formation and cell membrane directed flow of core particles in chronic hepatitis B","volume":"73","author":"Gudat","year":"1977","journal-title":"Gastroenterology"},{"key":"10.1002\/hep.1840040111-BIB7","first-page":"1","article-title":"Immunohistologic demonstration of hepatitis B viral antigens in liver with reference to its significance in liver injury","volume":"40","author":"Huang","year":"1979","journal-title":"Lab Invest"},{"key":"10.1002\/hep.1840040111-BIB8","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/0016-5085(82)90007-5","article-title":"Core-antigen specific immunoglobulin G bound to the liver cell membrane in chronic hepatitis B","volume":"82","author":"Trevisan","year":"1982","journal-title":"Gastroenterology"},{"key":"10.1002\/hep.1840040111-BIB9","doi-asserted-by":"crossref","first-page":"2773","DOI":"10.4049\/jimmunol.129.6.2773","article-title":"Specificity of T lymphocyte cytotoxicity to autologous hepatocytes in chronic hepatitis B virus infection: evidence that T-cells are directed against HBV-core antigen expressed on hepatocytes","volume":"129","author":"Mondelli","year":"1982","journal-title":"J Immunol"},{"key":"10.1002\/hep.1840040111-BIB10","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1002\/jmv.1890060407","article-title":"Contrasting patterns and frequency of antibodies to the surface, core and e antigen of hepatitis B virus in blood donors and in homosexual patients","volume":"6","author":"Tedder","year":"1980","journal-title":"J Med Virol"},{"key":"10.1002\/hep.1840040111-BIB11","first-page":"16","article-title":"Lymphocyte cytotoxicity to autologous hepatocytes in HBsAg-negative chronic active hepatitis","volume":"38","author":"Mieli-Vergani","year":"1979","journal-title":"Clin Exp Immunol"},{"key":"10.1002\/hep.1840040111-BIB12","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/0022-1759(81)90114-9","article-title":"Immunohistochemistry with enzyme labelled antibodies. A brief review","volume":"17","author":"Farr","year":"1981","journal-title":"J Immunol Methods"},{"key":"10.1002\/hep.1840040111-BIB13","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1017\/S0022172400063932","article-title":"Production of monoclonal antibodies to hepatitis B surface and core antigens, and use in the detection of viral antigens in liver biopsies","volume":"90","author":"Tedder","year":"1983","journal-title":"J Hygiene"},{"key":"10.1002\/hep.1840040111-BIB14","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1177\/20.12.975","article-title":"Changes in the solubility of immunoglobulins after fluorescent labelling and its influence on immuno-fluorescent techniques","volume":"20","author":"Arnold","year":"1972","journal-title":"J Histochem Cytochem"},{"key":"10.1002\/hep.1840040111-BIB15","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1136\/gut.17.11.837","article-title":"Complement fixing hepatitis B core antigen immune complexes in the liver of patients with HBs antigen positive chronic disease","volume":"17","author":"Rizzetto","year":"1976","journal-title":"Gut"},{"key":"10.1002\/hep.1840040111-BIB16","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1136\/gut.23.12.1029","article-title":"Lymphocyte cytotoxicity to autologous hepatocytes in HBsAg positive chronic liver disease","volume":"23","author":"Mieli-Vergani","year":"1982","journal-title":"Gut"},{"key":"10.1002\/hep.1840040111-BIB17","first-page":"85S","article-title":"Hepatitis B virus DNA detection by in situ cytohybridization: implications for viral replication strategy and pathogenesis of chronic hepatitis","volume":"2","author":"Burrell","year":"1982","journal-title":"Hepatology"},{"key":"10.1002\/hep.1840040111-BIB18","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1016\/S0140-6736(81)90182-3","article-title":"Detection of hepatitis B virus DNA in liver and serum: a direct appraisal of the chronic carrier state","volume":"2","author":"Brechot","year":"1981","journal-title":"Lancet"},{"key":"10.1002\/hep.1840040111-BIB19","doi-asserted-by":"crossref","first-page":"683","DOI":"10.4049\/jimmunol.129.2.683","article-title":"Viral antigens act as helper determinants for antibody responses to cell surface antigens","volume":"129","author":"Bromberg","year":"1982","journal-title":"J Immunol"},{"key":"10.1002\/hep.1840040111-BIB20","doi-asserted-by":"crossref","first-page":"78","DOI":"10.4049\/jimmunol.125.1.78","article-title":"Alteration in expression of measles virus polypeptides by antibody: molecular events in antibody-induced antigenic modulation","volume":"125","author":"Fujinami","year":"1981","journal-title":"J Immunol"},{"key":"10.1002\/hep.1840040111-BIB21","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1007\/BF02905874","article-title":"Electron microscopy of isolated human hepatocytes: micromethods for scanning and transmission electron microscopy","volume":"13","author":"Trevisan","year":"1983","journal-title":"La Ricerca"},{"key":"10.1002\/hep.1840040111-BIB22","first-page":"170","volume-title":"Immunocytochemistry","author":"Sternberger","year":"1979"},{"key":"10.1002\/hep.1840040111-BIB23","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/0016-5085(79)90267-1","article-title":"Relationship between membrane-bound immunoglobulin and viral antigens in liver cells from patients with hepatitis B virus infection","volume":"77","author":"Trevisan","year":"1979","journal-title":"Gastroenterology"}],"container-title":["Hepatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fhep.1840040111","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.lww.com\/01515467-198401000-00011","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T11:16:12Z","timestamp":1733051772000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/01515467-198401000-00011"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1984,1]]},"references-count":23,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1984]]}},"URL":"https:\/\/doi.org\/10.1002\/hep.1840040111","relation":{},"ISSN":["0270-9139"],"issn-type":[{"value":"0270-9139","type":"print"}],"subject":[],"published":{"date-parts":[[1984,1]]}}}