{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T11:40:24Z","timestamp":1733053224102,"version":"3.30.0"},"reference-count":15,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":11200,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1985,1]]},"abstract":"\n \n Recent studies suggest that the serum level of Type III procollagen (PC\u2013III) could be a valuable, noninvasive monitor of hepatic fibrogenesis. We have developed a sensitive and specific radioimmunoassay for PC\u2013III procollagen isolated and purified from fetal goat skin which shows high cross\u2013reaction for human PC\u2013III. In a double\u2013blind study, serum samples taken from 8 normal volunteers and 50 patients at the time of liver biopsy were assayed for PC\u2013III. Each biopsy was reviewed without knowledge of the radioimmunoassay results, and old or active fibrosis was graded on a 0 to 3 (none, minimal, moderate, severe) scale. There was a significant difference between serum concentrations of PC\u2013III in normals (mean \u00b1 S.D. = 72 ng per ml \u00b1 8) as compared to patients with biopsy evidence of active fibrosis (mean \u00b1 S.D. = 153 ng per ml \u00b1 12; p < 0.001). The serum concentration of PC\u2013III showed a good correlation (r = 0.58; p < 0.001) with the histological grade of active lobular fibrosis. Measurement of PC\u2013III is a noninvasive test for detection of active fibrosis in the liver.<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/hep.1840050109","type":"journal-article","created":{"date-parts":[[2007,3,3]],"date-time":"2007-03-03T18:03:13Z","timestamp":1172944993000},"page":"38-42","update-policy":"http:\/\/dx.doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":41,"title":["A Radioimmunoassay Procedure for Type Iii Procollagen: Its Use in the Detection of Hepatic Fibrosis"],"prefix":"10.1097","volume":"5","author":[{"given":"Michael R.","family":"Galambos","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Delwood C.","family":"Collins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John T.","family":"Galambos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"10.1002\/hep.1840050109-BIB1","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1016\/S0016-5085(79)80170-5","volume":"76","author":"Rojkind","year":"1979","journal-title":"Gastroenterology"},{"key":"10.1002\/hep.1840050109-BIB2","first-page":"142","volume-title":"Frontiers in liver disease","author":"Stern","year":"1981","unstructured":". The fibrosis of chronic alcoholic liver disease. In: , eds. Frontiers in liver disease. New York: Georg Thieme Verlag, 1981: 142\u2013155."},{"key":"10.1002\/hep.1840050109-BIB3","doi-asserted-by":"crossref","first-page":"2980","DOI":"10.1021\/bi00632a027","volume":"16","author":"Smith","year":"1977","journal-title":"Biochemistry"},{"key":"10.1002\/hep.1840050109-BIB4","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/0022-1759(76)90141-1","volume":"11","author":"Rohde","year":"1976","journal-title":"J Immunol Methods"},{"key":"10.1002\/hep.1840050109-BIB5","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1111\/j.1365-2362.1979.tb00912.x","volume":"9","author":"Rohde","year":"1979","journal-title":"Eur J Clin Invest"},{"key":"10.1002\/hep.1840050109-BIB6","first-page":"205","volume-title":"Connective tissue of the normal and fibrotic human liver","author":"Hahn","year":"1982","unstructured":", , et al. Radioimmunologic determination of procollagen Type III peptide in sera of patients with chronic liver disease: correlation with clinical and histologic data. In: , , et al. eds. Connective tissue of the normal and fibrotic human liver. New York: Thieme Stratton, Inc., 1982: 205\u2013206."},{"key":"10.1002\/hep.1840050109-BIB7","first-page":"212","volume-title":"Connective tissue of the normal and fibrotic human liver","author":"Penceu","year":"1982","unstructured":", , et al. Discriminant analysis of laboratory, histological, and serum procollagen peptide data in patients with acute and chronic liver disease. In: , , et al. eds. Connective tissue of the normal and fibrotic human liver. New York: Thieme Stratton, Inc., 1982: 212\u2013214."},{"key":"10.1002\/hep.1840050109-BIB8","first-page":"1566","volume":"40","author":"Galambos","year":"1981","journal-title":"Fed Proc"},{"key":"10.1002\/hep.1840050109-BIB9","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1002\/hep.1840050109-BIB10","volume-title":"Immunoassays for the 80's","author":"McConaley","year":"1981","unstructured":". In: , , eds. Immunoassays for the 80's. Baltimore: University Park Press, 1981."},{"key":"10.1002\/hep.1840050109-BIB11","first-page":"204","volume-title":"Principles of competitive protein binding assays","author":"Rodbard","year":"1971","unstructured":"In: , eds. Principles of competitive protein binding assays. Philadelphia: W. B. Lippincott Co., 1971: 204."},{"key":"10.1002\/hep.1840050109-BIB12","first-page":"431","volume-title":"Applied regression analysis and other multivariable methods, North Scituate","author":"Kleinbaum","year":"1978","unstructured":". In Applied regression analysis and other multivariable methods, North Scituate. Massachusetts: Massachusetts Duxbury Press, 1978: 431."},{"key":"10.1002\/hep.1840050109-BIB13","first-page":"217","volume-title":"Connective tissue of the normal and fibrotic human liver","author":"Pierard","year":"1982","unstructured":", , et al. Type III collagen precursor sequences in sera of patients with hepatic diseases. In: , , et al. eds. Connective tissue of the normal and fibrotic human liver. New York: Thieme Stratton, Inc., 1982: 217\u2013218."},{"key":"10.1002\/hep.1840050109-BIB14","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/0016-5085(83)90308-6","volume":"85","author":"Neimela","year":"1983","journal-title":"Gastroenterology"},{"key":"10.1002\/hep.1840050109-BIB15","first-page":"801","volume-title":"The liver: biology and pathobiology","author":"Rojking","year":"1983","unstructured":"Fibrogenesis. In: , et al. eds. The liver: biology and pathobiology. New York: Raven Press, 1983: 801\u2013809."}],"container-title":["Hepatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fhep.1840050109","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.lww.com\/01515467-198501000-00008","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T11:10:26Z","timestamp":1733051426000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/01515467-198501000-00008"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,1]]},"references-count":15,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1985]]}},"URL":"https:\/\/doi.org\/10.1002\/hep.1840050109","relation":{},"ISSN":["0270-9139"],"issn-type":[{"type":"print","value":"0270-9139"}],"subject":[],"published":{"date-parts":[[1985,1]]}}}