{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T03:09:15Z","timestamp":1761620955221,"version":"3.30.0"},"reference-count":38,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":10957,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1985,9]]},"abstract":"<jats:sec>\n            <jats:title\/>\n            <jats:p>Twenty patients with HBeAg\u2013positive chronic liver disease were given large doses of recombinant leukocyte interferon for 4 weeks. Changes of hepatitis B virus DNA in livers and sera were analyzed by the molecular hybridization technique in paired biopsies obtained before and 2 weeks after treatment. Serum hepatitis B virus DNA was examined before, during and after the treatment until 4 weeks post\u2013interferon.<\/jats:p>\n            <jats:p>Analysis of hepatic hepatitis B virus DNA revealed species that appeared to represent various forms of replicative hepatitis B virus DNA, i.e., relaxed circular, linear, supercoiled and single\u2013stranded hepatitis B virus DNA, respectively. No evidence of integration of hepatitis B virus DNA in genomic DNA was obtained. Of 15 cases which were positive for hepatic hepatitis B virus DNA before treatment and in which paired biopsies were obtained, hepatic hepatitis B virus DNA became negative in 4, decreased in 5 and unchanged in 6. Among several types of replicative viral DNA in liver tissue, supercoiled hepatitis B virus DNA tended to remain after other forms were reduced.<\/jats:p>\n            <jats:p>A close correlation between hepatic and serum hepatitis B virus DNA was found in 37 liver biopsy samples and corresponding sera.<\/jats:p>\n            <jats:p>These results indicate that interferon treatment reduces serum hepatitis B virus levels by inhibiting viral replication in the liver and that persistence or reappearance of hepatitis B virus in serum after interferon is associated with replication.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1002\/hep.1840050505","type":"journal-article","created":{"date-parts":[[2007,3,3]],"date-time":"2007-03-03T19:13:55Z","timestamp":1172949235000},"page":"728-734","update-policy":"http:\/\/dx.doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":80,"title":["Changes of Hepatitis B Virus Dna in Liver and Serum Caused by Recombinant Leukocyte Interferon Treatment: Analysis of Intrahepatic Replicative Hepatitis B Virus Dna"],"prefix":"10.1097","volume":"5","author":[{"given":"Osamu","family":"Yokosuka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masao","family":"Omata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fumio","family":"Imazeki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kunio","family":"Okuda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jesse","family":"Summers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"10.1002\/hep.1840050505-BIB1","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1126\/science.6254146","volume":"210","author":"Maugh","year":"1980","journal-title":"Science"},{"key":"10.1002\/hep.1840050505-BIB2","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1056\/NEJM197610212951701","volume":"295","author":"Alter","year":"1976","journal-title":"N 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