{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:24:00Z","timestamp":1762100640868,"version":"3.30.0"},"reference-count":57,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":10776,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1986,3]]},"abstract":"<jats:sec>\n            <jats:title\/>\n            <jats:p>Specific transcriptional and translational products associated with regenerating liver were analyzed by differential hybridization to a cDNA library and by two\u2013dimensional electrophoresis of hepatic proteins, respectively. Comparisons of approximately 800 soluble and 800 particulate liver proteins from normal and 70% partially hepatectomized Fischer rats resulted in the identification of only three apparently unique polypeptides in 70% partially hepatectomized livers, although many quantitative changes were observed. A subset of these quantitative changes were also observed after sham operation. A cDNA library was generated from polyadenylated RNA isolated 18 hr post\u201370% partial hepatectomy. Comparative analysis of 6,000 transformantswith single\u2013stranded cDNA probes prepared from 18 hr post\u201370% partial hepatectomy and sham\u2013operated animals identified three clones whose sequences were preferentially expressed 4\u2013 to 6\u2013fold 18 hr post\u201370%partial hepatectomy. Southern blot analysis of one clone, REG\u2013A, showed no homology to albumin, \u03b1\u2013fetoprotein, three different forms of cytochrome P\u2013450, ornithine decarboxylase, globin, or to a putative tumor promotion associated gene called PRO\u20132. A single, REG\u2013A specific 2.5 kb band was identified by Northern blot analysis of liver samples. REG\u2013A expression was increased 2\u2013fold 18 hr postsham operation; 4\u2013fold 18 hr post\u201370% partialhepatectomy and following chronic 2,3,7,8\u2013tetrachlorodibenzo\u2013<jats:italic toggle=\"yes\">p<\/jats:italic>\u2013dioxin or phenobarbital treatment. REG\u2013A expression returned to control levels 1 week after 70% partial hepatectomy. Furthermore, expression of REG\u2013Awas reduced in chemically induced preneoplastic nodules and in primary and transplantable hepatomas. Hybrid selection studies indicated that the REG\u2013A sequence selected a mRNA(s) species, that in an <jats:italic toggle=\"yes\">in vitro<\/jats:italic> translation assay, produced two major polypeptides of 21,000 and 25,000 molecular weight with a pI of 6.9.<\/jats:p>\n            <jats:p>Thus, these data support the hypothesis that liver regeneration is characterized byquantitative changes in genes normally expressed at low levels in the Go hepatocyte andis not the result of major qualitative changes in gene expression.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1002\/hep.1840060210","type":"journal-article","created":{"date-parts":[[2007,3,3]],"date-time":"2007-03-03T19:36:51Z","timestamp":1172950611000},"page":"209-219","update-policy":"http:\/\/dx.doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":18,"title":["Studies of Gene Transcription and Translation in Regenerating Rat Liver"],"prefix":"10.1097","volume":"6","author":[{"given":"Brian E.","family":"Huber","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carole A.","family":"Heilman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter J.","family":"Wirth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark J.","family":"Miller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Snorri S.","family":"Thorgeirsson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"10.1002\/hep.1840060210-BIB1","volume-title":"Regeneration of liver and kidney","author":"Bucher","year":"1971","unstructured":". Regeneration of liver and kidney. Boston, Massachusetts: Little, Brown and Co., 1971."},{"key":"10.1002\/hep.1840060210-BIB2","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1007\/BF00231309","volume":"59","author":"Fausto","year":"1984","journal-title":"Molec Cell Biochem"},{"key":"10.1002\/hep.1840060210-BIB3","first-page":"842","volume":"22","author":"Grisham","year":"1962","journal-title":"Cancer Res"},{"key":"10.1002\/hep.1840060210-BIB4","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1021\/bi00574a025","volume":"18","author":"Atryzek","year":"1979","journal-title":"Biochemistry"},{"key":"10.1002\/hep.1840060210-BIB5","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1016\/S0022-2836(67)80118-9","volume":"23","author":"Church","year":"1967","journal-title":"J Molec Biol"},{"key":"10.1002\/hep.1840060210-BIB6","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1016\/0005-2787(78)90305-2","volume":"521","author":"Tedeschi","year":"1978","journal-title":"Biochim Biophys Acta"},{"key":"10.1002\/hep.1840060210-BIB7","doi-asserted-by":"crossref","first-page":"2855","DOI":"10.1016\/S0021-9258(19)85818-0","volume":"255","author":"Scholla","year":"1980","journal-title":"J. Biol Chem"},{"key":"10.1002\/hep.1840060210-BIB8","doi-asserted-by":"crossref","first-page":"2200","DOI":"10.1016\/S0021-9258(18)34906-8","volume":"257","author":"Fausto","year":"1982","journal-title":"J Biol Chem"},{"key":"10.1002\/hep.1840060210-BIB9","first-page":"186","volume":"12","author":"Higgins","year":"1931","journal-title":"Arch Pathol"},{"key":"10.1002\/hep.1840060210-BIB10","first-page":"320","volume-title":"Liver regeneration after experimental injury","author":"Heine","year":"1975","unstructured":". A hypothesis of growth regulation in liver and other stable organs. In: , eds. Liver regeneration after experimental injury. New York: Stratton Intercontinental Medical Book Corp., 1975: 320\u2013329."},{"key":"10.1002\/hep.1840060210-BIB11","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal Biochem"},{"key":"10.1002\/hep.1840060210-BIB12","first-page":"5552","volume":"43","author":"Huber","year":"1983","journal-title":"Cancer Res"},{"key":"10.1002\/hep.1840060210-BIB13","doi-asserted-by":"crossref","first-page":"4007","DOI":"10.1016\/S0021-9258(19)41496-8","volume":"250","author":"O'Farrell","year":"1975","journal-title":"J Biol Chem"},{"key":"10.1002\/hep.1840060210-BIB14","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1038\/263701a0","volume":"263","author":"Solt","year":"1976","journal-title":"Nature"},{"key":"10.1002\/hep.1840060210-BIB15","first-page":"595","volume":"88","author":"Solt","year":"1977","journal-title":"Am J 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Their synthesis, turnover and biological significance. In ed. Structure and function of plasma proteins Vol 1. 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