{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:23:44Z","timestamp":1762100624199,"version":"3.37.0"},"reference-count":28,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":9466,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1989,10]]},"abstract":"<jats:sec>\n            <jats:title\/>\n            <jats:p>Transport of taurocholate into the hepatocyte against unfavorable chemical and electrical gradients occurs via a sodium-dependent, carrier-mediated transport system. Although this cotransporter has been characterized in the rodent, it has not been demonstrated in man. Therefore, we utilized human liver, obtained via multiorgan donation but not used for transplantation, to prepare basolateral (sinusoidal) liver plasma membrane vesicles by a Percoll gradient method. Na<jats:sup>+<\/jats:sup>,K<jats:sup>+<\/jats:sup> -ATPase, a marker enzyme for the basolateral domain, was enriched 28.9-fold in the final membrane fraction compared with homogenate, whereas the bile canalicular membrane enzymes Mg<jats:sup>++<\/jats:sup> -ATPase and alkaline phosphatase were enriched only 3.4- and 6.4-fold, respectively. Marker enzyme activities for endoplasmic reticulum, lysosomes and mitochondria were not enriched compared with homogenate. Integrity of the membrane vesicles was confirmed by the demonstration of Na<jats:sup>+<\/jats:sup> -dependent concentrative uptake of the amino acid L-alanine (estimated intravesicular volume of 0.59 \u03bcl per mg protein). An inwardly directed 100 m<jats:italic toggle=\"yes\">M<\/jats:italic> Na<jats:sup>+<\/jats:sup> gradient stimulated the initial rate of 2.5 \u03bc<jats:italic toggle=\"yes\">M<\/jats:italic> taurocholate uptake and energized a transient 2-fold accumulation of the bile acid above equilibrium (\u201covershoot\u201d). In contrast, uptake was slower and no overshoot occurred with a K<jats:sup>+<\/jats:sup> gradient. A negative intravesicular potential, created by altering accompanying anions or by valinomycin-induced K<jats:sup>+<\/jats:sup> diffusion potentials, did not enhance taurocholate uptake, suggesting an electroneutral cotransport mechanism. Chloride as the accompanying anion stimulated the initial rate of uptake compared with anions of lesser or greater lipid permeability. Na<jats:sup>+<\/jats:sup> -dependent taurocholate (4 \u03bc<jats:italic toggle=\"yes\">M<\/jats:italic>) uptake was significantly inhibited by 250 \u03bc<jats:italic toggle=\"yes\">M<\/jats:italic> cholate, taurocholate, glycocholate, taurochenodeoxycholate and bromsulfophthalein. Conversely, in the presence of a K<jats:sup>+<\/jats:sup> gradient, only taurochenodeoxycholate and bromsulfophthalein significantly inhibited taurocholate uptake. The initial rate (5 sec) of Na<jats:sup>+<\/jats:sup> -dependent taurocholate uptake, measured as a function of extravesicular taurocholate concentration (1 to 200 \u03bc<jats:italic toggle=\"yes\">M<\/jats:italic>), demonstrated Michaelis-Menten kinetics with a K<jats:sub>m<\/jats:sub> of 33.8 \u00b1 4.4 \u03bc<jats:italic toggle=\"yes\">M<\/jats:italic> and a V<jats:sub>max<\/jats:sub> of 1.25 \u00b1 0.99 nmoles per mg protein per min. We conclude from these studies that taurocholate uptake by basolateral plasma membrane vesicles from human liver is sodium dependent, electroneutral, inhibitable by other bile acids and saturable, with an apparent K<jats:sub>m<\/jats:sub> near the concentration of taurocholate found in human portal blood.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1002\/hep.1840100408","type":"journal-article","created":{"date-parts":[[2007,3,3]],"date-time":"2007-03-03T22:26:42Z","timestamp":1172960802000},"page":"447-453","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":36,"title":["Taurocholate transport by basolateral plasma membrane vesicles isolated from human liver"],"prefix":"10.1097","volume":"10","author":[{"given":"Donald A.","family":"Novak","sequence":"first","affiliation":[]},{"given":"Frederick C.","family":"Ryckman","sequence":"additional","affiliation":[]},{"given":"Frederick J.","family":"Suchy","sequence":"additional","affiliation":[]}],"member":"276","reference":[{"key":"10.1002\/hep.1840100408-BIB1","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1016\/0016-5085(82)90027-0","volume":"82","author":"Blitzer","year":"1982","journal-title":"Gastroenterology"},{"key":"10.1002\/hep.1840100408-BIB2","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1042\/bj2440249","volume":"244","author":"Coleman","year":"1987","journal-title":"Biochem J"},{"key":"10.1002\/hep.1840100408-BIB3","first-page":"181","volume":"359","author":"Anwer","year":"1978","journal-title":"Hoppe-Seyler's Z Physiol Chem"},{"key":"10.1002\/hep.1840100408-BIB4","first-page":"g484","volume":"243","author":"Van Dyke","year":"1982","journal-title":"Am J Physiol"},{"key":"10.1002\/hep.1840100408-BIB5","doi-asserted-by":"crossref","first-page":"1470","DOI":"10.1172\/JCI111103","volume":"71","author":"Duffy","year":"1983","journal-title":"J Clin Invest"},{"key":"10.1002\/hep.1840100408-BIB6","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1002\/hep.1840020510","volume":"2","author":"Inoue","year":"1982","journal-title":"Hepatology"},{"key":"10.1002\/hep.1840100408-BIB7","doi-asserted-by":"crossref","first-page":"10614","DOI":"10.1016\/S0021-9258(18)91006-9","volume":"259","author":"Meier","year":"1984","journal-title":"J Biol Chem"},{"key":"10.1002\/hep.1840100408-BIB8","doi-asserted-by":"crossref","first-page":"9295","DOI":"10.1016\/S0021-9258(17)47298-X","volume":"259","author":"Blitzer","year":"1984","journal-title":"J Biol Chem"},{"key":"10.1002\/hep.1840100408-BIB9","first-page":"790","volume":"93","author":"Scharschmidt","year":"1979","journal-title":"J Lab Clin Med"},{"key":"10.1002\/hep.1840100408-BIB10","doi-asserted-by":"crossref","first-page":"1590","DOI":"10.1172\/JCI109620","volume":"64","author":"Keeffe","year":"1979","journal-title":"J Clin Invest"},{"key":"10.1002\/hep.1840100408-BIB11","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/0076-6879(67)10042-6","volume":"10","author":"Tisdale","year":"1967","journal-title":"Methods Enzymol"},{"key":"10.1002\/hep.1840100408-BIB12","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1083\/jcb.32.2.415","volume":"32","author":"Sottocasa","year":"1967","journal-title":"J Cell Biol"},{"key":"10.1002\/hep.1840100408-BIB13","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1083\/jcb.57.3.630","volume":"57","author":"Rothstein","year":"1973","journal-title":"J Cell Biol"},{"key":"10.1002\/hep.1840100408-BIB14","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","volume":"193","author":"Lowry","year":"1951","journal-title":"J Biol Chem"},{"key":"10.1002\/hep.1840100408-BIB15","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/0076-6879(79)63008-2","volume":"63","author":"Cleland","year":"1979","journal-title":"Methods Enzymol"},{"key":"10.1002\/hep.1840100408-BIB16","volume-title":"Statistical methods","author":"Snedecor","year":"1967","unstructured":". Statistical methods, Ed. 6. Ames, Iowa: Iowa State University Press, 1967."},{"key":"10.1002\/hep.1840100408-BIB17","first-page":"g648","volume":"248","author":"Suchy","year":"1985","journal-title":"Am J Physiol"},{"key":"10.1002\/hep.1840100408-BIB18","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1083\/jcb.42.1.68","volume":"42","author":"Weibel","year":"1969","journal-title":"J Cell Biol"},{"key":"10.1002\/hep.1840100408-BIB19","doi-asserted-by":"crossref","first-page":"16060","DOI":"10.1016\/S0021-9258(18)66676-1","volume":"261","author":"Turner","year":"1986","journal-title":"J Biol Chem"},{"key":"10.1002\/hep.1840100408-BIB20","doi-asserted-by":"crossref","first-page":"1236","DOI":"10.1021\/bi00274a038","volume":"22","author":"Radian","year":"1983","journal-title":"Biochemistry"},{"key":"10.1002\/hep.1840100408-BIB21","doi-asserted-by":"crossref","first-page":"1333","DOI":"10.1016\/0006-2952(81)90317-8","volume":"30","author":"Laperche","year":"1981","journal-title":"Biochem Pharmacol"},{"key":"10.1002\/hep.1840100408-BIB22","first-page":"1027","volume":"359","author":"Anwer","year":"1978","journal-title":"Hoppe-Seyler's Z Physiol Chem"},{"key":"10.1002\/hep.1840100408-BIB23","first-page":"443","volume":"87","author":"Ware","year":"1976","journal-title":"J Lab Clin Med"},{"key":"10.1002\/hep.1840100408-BIB24","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/0005-2736(84)90015-4","volume":"769","author":"Zeigler","year":"1984","journal-title":"Biochim Biophys Acta"},{"key":"10.1002\/hep.1840100408-BIB25","first-page":"125","volume-title":"Progress in liver diseases","author":"Berk","year":"1986","unstructured":". Hepatocellular uptake of organic anions. In: , eds. Progress in liver diseases, Vol. VII. New York: Grune and Stratton, 1986; 125\u2013144."},{"key":"10.1002\/hep.1840100408-BIB26","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1111\/j.1432-1033.1976.tb10287.x","volume":"64","author":"Schwenk","year":"1976","journal-title":"Eur J Biochem"},{"key":"10.1002\/hep.1840100408-BIB27","doi-asserted-by":"crossref","first-page":"12042","DOI":"10.1016\/S0021-9258(18)67199-6","volume":"261","author":"Blitzer","year":"1986","journal-title":"J Biol Chem"},{"key":"10.1002\/hep.1840100408-BIB28","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1172\/JCI110668","volume":"70","author":"Angelin","year":"1982","journal-title":"J Clin Invest"}],"container-title":["Hepatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fhep.1840100408","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.lww.com\/01515467-198910000-00007","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,7]],"date-time":"2025-02-07T15:25:03Z","timestamp":1738941903000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/01515467-198910000-00007"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,10]]},"references-count":28,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1989]]}},"URL":"https:\/\/doi.org\/10.1002\/hep.1840100408","relation":{},"ISSN":["0270-9139"],"issn-type":[{"type":"print","value":"0270-9139"}],"subject":[],"published":{"date-parts":[[1989,10]]}}}