{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T16:30:53Z","timestamp":1697992253939},"reference-count":22,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,1,15]],"date-time":"2013-01-15T00:00:00Z","timestamp":1358208000000},"content-version":"vor","delay-in-days":10425,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hematological Oncology"],"published-print":{"date-parts":[[1984,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Treatment of P3HR\u20101 Burkitt's lymphoma cultured cells with 4 mM <jats:italic>n<\/jats:italic>\u2010butyrate for 48 h induced the abundant expression of a 35 000 dalton, microsomal membrane protein which was demonstrated to be the electrophoretically invariant Ii molecule. To prove this point, [<jats:sup>35<\/jats:sup>S]methionine\u2010labelled, microsomal membrane proteins or immunoprecipitates of such detergent\u2010solubilized proteins were subjected to electrophoresis either in SDS gels or in two\u2010dimensional, nonequilibrium pH gradient gels. The induced p35 molecule was judged to be Ii by molecular weight and isoelectric characteristics and by immune complex associations. The abundant expression of Ii on butyrate\u2010treated P3HR\u20101 cells mimicked the high level of expression of p35 or Ii on leukemic cells of a subset of patients with hairy cell leukemia. Alpha and beta chains of the HLA\u2010D complex were absent from the P3HR\u20101 cell line and present in the Jijoye parent from which mutant P3HR\u20101 was derived. This relatively simple model can lead to the study of inducible synthesis, processing and expression of Ii, with or without HLA\u2010D alpha and beta chains.<\/jats:p>","DOI":"10.1002\/hon.2900020303","type":"journal-article","created":{"date-parts":[[2013,1,15]],"date-time":"2013-01-15T10:39:39Z","timestamp":1358246379000},"page":"239-247","source":"Crossref","is-referenced-by-count":5,"title":["Enhanced Ii expression after <i>n<\/i>\u2010butyrate treatment of a P3HR\u20101 Burkitt's lymphoma subline which does not express HLA\u2010D"],"prefix":"10.1002","volume":"2","author":[{"given":"Robert C.","family":"Spiro","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Sairenji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert E.","family":"Humphreys","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,15]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1128\/iai.39.1.85-90.1983","article-title":"p105, an Epstein\u2010Barr virus\u2010induced, phosphonoacetic acid\u2010insensitive glycoprotein target of the anti\u2010Epstein\u2010Barr virus immune response","volume":"39","author":"Casareale D.","year":"1983","journal-title":"Infect. Immun."},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000149530"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(83)90101-3"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.12.6567"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.158.4.1344"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/294266a0"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.1.5.1045-1051.1967"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.144.1.98"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(79)90027-0"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.132.1.12"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(82)90090-3"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(79)90455-0"},{"key":"e_1_2_1_14_1","doi-asserted-by":"crossref","first-page":"2564","DOI":"10.4049\/jimmunol.129.6.2564","article-title":"Biosynthesis and glycosylation of the invariant chain associated with HLA\u2010DR antigens","volume":"129","author":"Machamer C. E.","year":"1982","journal-title":"J. Immunol."},{"key":"e_1_2_1_15_1","first-page":"4782","article-title":"Variable expression of membrane proteins among murine lymphoblastoid tumors as seen with sodium dodecylsulfate\u2010polyacrylamide gradient gel electrophoresis of [35S]methionine\u2010labelled cell membranes","volume":"39","author":"Malenka D. J.","year":"1979","journal-title":"Cancer Res."},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(77)90176-3"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1983.tb01507.x"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(80)90326-8"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/hon.2900010307"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0145-2126(80)90029-6"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0145-2126(84)90031-6"},{"key":"e_1_2_1_22_1","unstructured":"Spiro R. C. Sairenji T. Townsend P. Reisert P. S. Humphreys R. E.(1984b).The Ii nature of p35 in hairy leukemic cells.Semin. Oncol. in press."},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(81)90013-4"}],"container-title":["Hematological Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fhon.2900020303","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/hon.2900020303","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T20:49:12Z","timestamp":1697834952000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/hon.2900020303"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1984,7]]},"references-count":22,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1984,7]]}},"alternative-id":["10.1002\/hon.2900020303"],"URL":"https:\/\/doi.org\/10.1002\/hon.2900020303","archive":["Portico"],"relation":{},"ISSN":["0278-0232","1099-1069"],"issn-type":[{"value":"0278-0232","type":"print"},{"value":"1099-1069","type":"electronic"}],"subject":[],"published":{"date-parts":[[1984,7]]}}}