{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T01:45:54Z","timestamp":1772761554311,"version":"3.50.1"},"reference-count":48,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,5,17]],"date-time":"2005-05-17T00:00:00Z","timestamp":1116288000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2005,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Fibroblasts, which are a major component of cancer\u2010induced stroma, can have a significant impact on the progression of adjacent malignant epithelia. To characterize fibroblasts recruited into cancer\u2010induced stroma, we examined the recruitment efficiency of 9 human fibroblast cell lines into experimental tumors generated in immunodeficient mice. Green fluorescence protein (GFP)\u2013labeled fibroblast cell lines and human pancreatic cancer cell line Capan\u20101 were injected i.p. at different sites; the GFP\u2010labeled cells within xenografts were then analyzed. KM104<jats:sup>GFP<\/jats:sup> (bone marrow) and VA\u201013<jats:sup>GFP<\/jats:sup> (lung) were selectively recruited into cancer stroma more efficiently than the other cell lines. KM104<jats:sup>GFP<\/jats:sup> cells did not affect tumor volume; however, VA\u201013<jats:sup>GFP<\/jats:sup> cells increased tumor volume by about 2\u2010fold. After 5 cyclic <jats:italic>in vivo<\/jats:italic> passages of KM104<jats:sup>GFP<\/jats:sup> in Capan\u20101, we selected a subpopulation with an 8.4\u2010fold higher recruitment efficiency (KM104<jats:sup>GFP<\/jats:sup>\u20105G) compared to parental KM104<jats:sup>GFP<\/jats:sup>. KM104<jats:sup>GFP<\/jats:sup>\u20105G also exhibited higher chemotaxis and chemoinvasion activity compared to KM104<jats:sup>GFP<\/jats:sup> in response to cancer\u2010released chemoattractant(s). Oligonucleotide microarray analysis identified 8 genes with &gt;3\u2010fold upregulation and 6 genes with &gt;3\u2010fold downregulation in KM104<jats:sup>GFP<\/jats:sup>\u20105G. Immunohistochemistry confirmed that fibroblasts recruited into pancreatic cancer stroma strongly expressed carbonic anhydrase IX and keratin\u20108, whose transcripts were upregulated in KM104<jats:sup>GFP<\/jats:sup>\u20105G by oligonucleotide microarray analysis, whereas their expression in fibroblasts within noncancerous pancreatic stroma were under the detection level. Our results indicate that fibroblast recruitment is not selective with respect to organ origin and that particular fibroblast subpopulations with specific phenotypic characteristics could be recruited efficiently into cancer\u2010induced stroma. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.21199","type":"journal-article","created":{"date-parts":[[2005,5,17]],"date-time":"2005-05-17T18:46:52Z","timestamp":1116355612000},"page":"212-220","source":"Crossref","is-referenced-by-count":37,"title":["<i>In vivo<\/i> and <i>in vitro<\/i> characterization of human fibroblasts recruited selectively into human cancer 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