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We could distinguish cell lines showing strong transmigration (TEM+ cell lines) from others that did not transmigrate (TEM\u2212 cell lines). By DNA microarray analysis we could cluster TEM+ and TEM\u2212 cell lines according to their gene expression pattern and identify genes differentially expressed between the 2 groups. Among these we found the integrin \u03b23 subunit to be highly expressed in TEM+ cell lines as compared to TEM\u2212 cell lines. Cell surface localization of \u03b1v\u03b23 integrin receptors was exclusively found in TEM+ cell lines. Transendothelial migration of TEM+ cells but not their adhesion to the endothelial cells, or invasion into collagen gels could be blocked with an antibody against \u03b1v\u03b23 integrin and by RNAi mediated knock\u2010down of the integrin \u03b23 subunit. These data establishes \u03b1v\u03b23 integrin as one key component of the transendothelial migration process of tumor cells, and as a potential target for anti\u2010metastatic therapy. Our gene expression analysis of a defined collection of tumor cell lines can be used as a starting point to identify further genes functionally involved in transendothelial migration. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.22879","type":"journal-article","created":{"date-parts":[[2007,7,9]],"date-time":"2007-07-09T18:21:47Z","timestamp":1184005307000},"page":"1910-1918","source":"Crossref","is-referenced-by-count":51,"title":["Expression profiling reveals genes associated with transendothelial migration of tumor cells: A functional role for \u03b1v\u03b23 integrin"],"prefix":"10.1002","volume":"121","author":[{"given":"Katja","family":"Bauer","sequence":"first","affiliation":[]},{"given":"Claudia","family":"Mierke","sequence":"additional","affiliation":[]},{"given":"J\u00fcrgen","family":"Behrens","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2007,7,9]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.108.6.2435"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/32433"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc727"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.98.4.1853"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2005.01.006"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-0691"},{"key":"e_1_2_6_8_2","first-page":"6714","article-title":"The CD24\/P\u2010selectin binding pathway initiates lung arrest of human A125 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